Effect of cutting the zona pellucida on the pronuclear transplantation in the mouse.
Effect of cutting the zona pellucida on the pronuclear transplantation in the mouse.
复制标题
切断透明带对小鼠原核移植的影响。
DOI：
10.1002/jez.1402400115
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Sugie
中科院分区：
文献类型：
--
作者：
Y. Tsunoda;T. Yasui;K. Nakamura;T. Uchida;T. Sugie
McGrath 和 Solter ('83) 开发了一种新的、可靠的小鼠卵子原核移植程序。为了克服这种程序的技术困难，特别是在均匀准备去核移液器和减少显微操作过程中的损伤方面，我们研究了切割卵透明带的效果。通过在卵子的透明带上切开一条缝，可以缩短卵子之间移液和原核交换的时间，因为不需要移液器的锋利尖端。虽然培养1天或3天的原核移植卵移植后获得的妊娠受者和幼仔的比例较低，但培养4天的卵子移植后，显着增加（妊娠率70％，幼仔32％）。这些值与将培养的未手术卵转移至桑葚和囊胚后的值相当。
A new and reliable pronuclear transplantation procedure for the mouse egg has been developed by McGrath and Solter ('83). To overcome the technical difficulties of such a procedure, especially in uniformly preparing enucleation pipettes and in reducing damages during micromanipulation, we have examined the effect of cutting the zona pellucida of the eggs. By making a slit in the zona of an egg, the time for pipetting and exchange of pronuclei between eggs was shortened because the sharp tip of the pipette was not necessary. Although the proportion of pregnant recipients and young obtained after transfer of pronuclear transplanted eggs cultured for 1 day or 3 days was quite low, it was significantly increased (70% for pregnancy rate and 32% for the young) following transfer of eggs cultured for 4 days. These values were comparable with those after transfer of unoperated eggs cultured to morulae and blastocysts.
影响因子：
56.9
作者：
MCGRATH, J;SOLTER, D
通讯作者：
SOLTER, D