Visualization of Tau–Tubulin Interaction in a Living Cell Using Bifluorescence Complementation Technique
Visualization of Tau–Tubulin Interaction in a Living Cell Using Bifluorescence Complementation Technique
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使用双荧光互补技术可视化活细胞中 Tau-微管蛋白相互作用
DOI：
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发表时间：
2018
影响因子：
5.6
通讯作者：
Y. Kim
中科院分区：
文献类型：
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作者：
Seulgi Shin;Sungsu Lim;Hyeanjeong Jeong;Liam Kwan;Y. Kim
Tau是一种稳定微管的神经元特异性微管结合蛋白。通常认为，高度磷酸化的tau从微管解离并成为不溶性聚集体，导致神经元变性。由于tau聚集在神经退行性疾病（包括阿尔茨海默病）中的意义，已经做出了巨大的努力来鉴定tau聚集过程。然而，在聚集过程中tau与微管蛋白的相互作用在很大程度上仍然未知。为了仔细研究tau-微管蛋白相互作用，我们生成了一个细胞模型，该模型能够使用双荧光互补（BiFC）技术可视化活细胞中的tau-微管蛋白相互作用。在诱导tau病理的不同化学刺激后，tau-微管蛋白BiFC细胞显示出显著增加的BiFC荧光水平，表明tau与微管蛋白聚集在一起。我们的研究结果表明，微管蛋白应被认为是一个关键组成部分的tau蛋白聚集过程。
Tau is a neuron-specific microtubule-binding protein that stabilizes microtubules. It is generally thought that highly phosphorylated tau dissociates from microtubules and becomes insoluble aggregates, leading to neuronal degeneration. Due to the implication of tau aggregation in neurodegenerative disorders, including Alzheimer’s disease, great efforts have been made to identify the tau aggregation process. However, tau interaction with tubulin during the aggregation process remains largely unknown. To scrutinize the tau-tubulin interaction, we generated a cell model that enables visualization of the tau-tubulin interaction in a living cell using the Bifluorescence Complementation (BiFC) Technique. Upon diverse chemical stimulation that induced tau pathology, tau-tubulin BiFC cells showed significantly increased levels of BiFC fluorescence, indicating that tau aggregates together with tubulin. Our results suggest that tubulin should be considered as a key component in the tau aggregation process.
DOI：
10.1073/pnas.1504081112
发表时间：
2015-06-16
影响因子：
11.1
作者：
Kadavath, Harindranath;Hofele, Romina V.;Zweckstetter, Markus
通讯作者：
Zweckstetter, Markus
影响因子：
19
作者：
Song, Yuyu;Brady, Scott T.
通讯作者：
Brady, Scott T.
影响因子：
3.4
作者：
Li, Xiao-Han;Rhoades, Elizabeth
通讯作者：
Rhoades, Elizabeth