Proteome-wide quantification and characterization of oxidation-sensitive cysteines in pathogenic bacteria.
Proteome-wide quantification and characterization of oxidation-sensitive cysteines in pathogenic bacteria.
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DOI：
10.1016/j.chom.2013.02.004
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发表时间：
2013-03-13
影响因子：
30.3
通讯作者：
He C
中科院分区：
文献类型：
--
作者：
Deng X;Weerapana E;Ulanovskaya O;Sun F;Liang H;Ji Q;Ye Y;Fu Y;Zhou L;Li J;Zhang H;Wang C;Alvarez S;Hicks LM;Lan L;Wu M;Cravatt BF;He C
活性和变构半胱氨酸的巯基氧化是一种广泛的调节性翻译后蛋白质修饰。病原菌，包括铜绿假单胞菌和金黄色葡萄球菌，使用调节性半胱氨酸氧化来响应和克服宿主环境中遇到的活性氧（ROS）。为了获得这两种病原体中氧化敏感性半胱氨酸的蛋白质组范围内的观点，我们采用了基于竞争活性的蛋白质分析方法来全局量化过氧化氢（H2 O2）与细菌蛋白质组中半胱氨酸的反应性。我们鉴定了约200种含有H2 O2敏感性半胱氨酸的蛋白质，包括代谢酶、转录因子和未表征的蛋白质。进一步的生物化学和遗传学研究确定了主群体感应调节器LasR中的氧化响应性半胱氨酸，以及乙醛脱氢酶ExaC、精氨酸脱亚胺酶ArcA和甘油醛3-磷酸脱氢酶GAPDH的氧化还原调节活性。综上所述，我们的数据表明，病原菌表现出复杂的，多层次的反应，包括代谢途径的快速适应氧化应激的挑战ROS。
Thiol group oxidation of active and allosteric cysteines is a widespread regulatory post-translational protein modification. Pathogenic bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus, use regulatory cysteine oxidation to respond to and overcome reactive oxygen species (ROS) encountered in the host environment. To obtain a proteome-wide view of oxidation-sensitive cysteines in these two pathogens, we employed a competitive activity-based protein profiling approach to globally quantify hydrogen peroxide (H2O2) reactivity with cysteines across bacterial proteomes. We identified ~200 proteins containing H2O2-sensitive cysteines, including metabolic enzymes, transcription factors, and uncharacterized proteins. Further biochemical and genetic studies identified an oxidation-responsive cysteine in the master quorum sensing regulator LasR, and redox-regulated activities for acetaldehyde dehydrogenase ExaC, arginine deiminase ArcA, and glyceraldehyde 3-phosphate dehydrogenase GAPDH. Taken together, our data indicate that pathogenic bacteria exhibit a complex, multi-layered response to ROS that includes the rapid adaption of metabolic pathways to oxidative stress challenge.
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影响因子：
--
作者：
Klomsiri, Chananat;Nelson, Kimberly J.;Bechtold, Erika;Soito, Laura;Johnson, Lynnette C.;Lowther, W. Todd;Ryu, Seong-Eon;King, S. Bruce;Furdui, Cristina M.;Poole, Leslie B.
通讯作者：
Poole, Leslie B.
影响因子：
3.2
作者：
Oinuma, Ken-Ichi;Greenberg, E. Peter
通讯作者：
Greenberg, E. Peter
DOI：
10.1073/pnas.102483199
发表时间：
2002-05-14
影响因子：
11.1
作者：
Fuangthong, M;Helmann, JD
通讯作者：
Helmann, JD
影响因子：
15
作者：
Amara, Neri;Mashiach, Roi;Meijler, Michael M.
通讯作者：
Meijler, Michael M.
影响因子：
5.4
作者：
Kannan, Shibichakravarthy;Audet, Aaron;Wu, Min
通讯作者：
Wu, Min