Genome-wide identification of AP2/EREBP in Fragaria vesca and expression pattern analysis of the FvDREB subfamily under drought stress.
Genome-wide identification of AP2/EREBP in Fragaria vesca and expression pattern analysis of the FvDREB subfamily under drought stress.
复制标题
在干旱胁迫下，fragaria vesca中AP2/EREBP的全基因组鉴定以及FVDREB亚科的表达模式分析。
DOI：
10.1186/s12870-021-03095-2
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发表时间：
2021-06-26
影响因子：
5.3
通讯作者：
Cheng ZM
中科院分区：
文献类型：
--
作者：
Dong C;Xi Y;Chen X;Cheng ZM
干旱是世界范围内的普遍现象。它也是影响草莓生长和品质的主要非生物因子之一。脱水反应元件结合蛋白（DREB）属于APETALA 2/乙烯反应元件结合蛋白（AP 2/EREBP）超家族，是植物中特有的转录因子，在植物的非生物胁迫反应中发挥重要作用。本研究共鉴定出119个AP 2/EREBP基因，其中AP 2/EREBP超家族又分为AP 2、RAV、ERF、DREB和soloist亚家族，分别含有18个、7个、61个、32个和1个成员。根据系统发育分析，DREB亚家族可进一步分为A-1 ~ A-6 6个亚家族。通过基因结构、保守基序、染色体定位和同线性分析，对FvDREB的特性进行了全面的研究。此外，转录组分析揭示了干旱胁迫下草莓植株中FvDREB基因的独特表达模式。干旱胁迫下，FvDREB 6基因A-2亚组在老叶中表达下调，在幼叶中表达上调。qRT-PCR分析发现，干旱胁迫下，A-2亚群FvDREB 8的表达量最高。通过对FvDREB 18基因的表达模式、系统发育关系、基序和启动子的分析，推测FvDREB 18可能在FvDREB 1和FvDREB 2的表达调控中发挥重要作用。我们的发现为FvDREB的特征和潜在功能提供了新的见解。这些FvDREB基因是草莓耐旱性改良的理想候选基因，值得进一步研究。在线版本包含补充材料，可通过10.1186/s12870-021-03095-2获得。
Drought is a common phenomenon worldwide. It is also one of the main abiotic factors that affect the growth and quality of strawberry. The dehydration-responsive element binding protein (DREB) members that belong to the APETALA2/ethylene-responsive element binding protein (AP2/EREBP) superfamily are unique transcription factors in plants that play important roles in the abiotic stress response. Here, a total of 119 AP2/EREBP genes were identified in Fragaria vesca, and the AP2/EREBP superfamily was divided into AP2, RAV, ERF, DREB, and soloist subfamilies, containing 18, 7, 61, 32, and one member(s), respectively. The DREB subfamily was further divided into six subgroups (A-1 to A-6) based on phylogenetic analysis. Gene structure, conserved motifs, chromosomal location, and synteny analysis were conducted to comprehensively investigate the characteristics of FvDREBs. Furthermore, transcriptome analysis revealed distinctive expression patterns among the FvDREB genes in strawberry plants exposed to drought stress. The expression of FvDREB6 of the A-2 subgroup was down-regulated in old leaves and up-regulated in young leaves in response to drought. Furthermore, qRT-PCR analysis found that FvDREB8 from the A-2 subgroup had the highest expression level under drought stress. Together, analyses with the expression pattern, phylogenetic relationship, motif, and promoter suggest that FvDREB18 may play a critical role in the regulation of FvDREB1 and FvDREB2 expression. Our findings provide new insights into the characteristics and potential functions of FvDREBs. These FvDREB genes should be further studied as they appear to be excellent candidates for drought tolerance improvement of strawberry. The online version contains supplementary material available at 10.1186/s12870-021-03095-2.
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