Proteases and caspase-like activity in the yeast Saccharomyces cerevisiae.
Proteases and caspase-like activity in the yeast Saccharomyces cerevisiae.
复制标题
酿酒酵母中的蛋白酶和半胱天冬酶样活性。
DOI：
10.1042/bst0391502
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发表时间：
2011
影响因子：
3.9
通讯作者：
M. Ramsdale
中科院分区：
文献类型：
--
作者：
Derek Wilkinson;M. Ramsdale
多种蛋白酶与酵母PCD（程序性细胞死亡）有关，包括metacaspase Mca1和分离酶Esp1、hta样丝氨酸蛋白酶Nma111、组织蛋白酶样丝氨酸羧肽酶和一系列空泡蛋白酶。蛋白酶体活性也被证明在决定细胞命运中具有重要作用，具有促和抗凋亡的作用。在酵母PCD刺激下检测到Caspase 3-， 6-和8-样活性，但并非所有这些活性都与Mca1相关，这意味着在酵母细胞死亡反应中还有其他具有Caspase样活性的蛋白酶。本文讨论了伴随PCD的全局蛋白水解事件，并考虑了死亡相关降解物的保存（在底物选择和裂解位点水平上）。强调了降解组中功能获得变化和功能丧失变化的重要性。更好地了解死亡相关蛋白酶及其底物可能有助于设计未来的抗真菌药物或操纵工业酵母进行商业开发。
A variety of proteases have been implicated in yeast PCD (programmed cell death) including the metacaspase Mca1 and the separase Esp1, the HtrA-like serine protease Nma111, the cathepsin-like serine carboxypeptideases and a range of vacuolar proteases. Proteasomal activity is also shown to have an important role in determining cell fate, with both pro- and anti-apoptotic roles. Caspase 3-, 6- and 8-like activities are detected upon stimulation of yeast PCD, but not all of this activity is associated with Mca1, implicating other proteases with caspase-like activity in the yeast cell death response. Global proteolytic events that accompany PCD are discussed alongside a consideration of the conservation of the death-related degradome (both at the level of substrate choice and cleavage site). The importance of both gain-of-function changes in the degradome as well as loss-of-function changes are highlighted. Better understanding of both death-related proteases and their substrates may facilitate the design of future antifungal drugs or the manipulation of industrial yeasts for commercial exploitation.
DOI：
10.1016/j.bbamcr.2008.08.003
发表时间：
2009-04
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Li SC;Kane PM
通讯作者：
Kane PM
影响因子：
10.5
作者：
Fannjiang, Y;Cheng, WC;Hardwick, JM
通讯作者：
Hardwick, JM
半胱天冬酶样蛋白酶 Esp1 对 Mcd1 的裂解可促进芽殖酵母细胞凋亡。
影响因子：
3.3
作者：
Yang,Hui;Ren,Qun;Zhang,Zhaojie
通讯作者：
Zhang,Zhaojie