Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine.
Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine.
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DOI：
10.1016/j.bbrc.2012.09.144
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发表时间：
2012-11-09
影响因子：
3.1
通讯作者：
Yamashita, Akio
中科院分区：
文献类型：
--
作者：
Abe, Kaito;Ishigami, Tomoaki;Shyu, Ann-Bin;Ohno, Shigeo;Umemura, Satoshi;Yamashita, Akio
干扰素-β (IFN-β)是一种重要的抗病毒细胞因子，对先天和获得性免疫应答病原体至关重要。多肌苷：多胞酸（poly(I:C)）处理诱导IFN-β mRNA的短暂积累，这涉及IFN-β mRNA的增加和减少。这一现象已被广泛分析，作为理解基因诱导响应外部刺激机制的模型。利用一种新的RNA代谢标记方法与乙基尿苷直接测量从头RNA合成和RNA稳定性，我们重新评估了IFN-β mRNA的从头合成和降解。我们发现，在poly（I:C）处理长生不老的人支气管上皮细胞后，IFN-β mRNA的最大积累后，转录活性保持不变。我们还观察到IFN-β mRNA在最大积累前后的稳定性发生了意想不到的变化。结果表明，这种RNA代谢标记方法为同时分析转录活性和mRNA稳定性以及转录时间提供了一种通用方法。
Interferon-beta (IFN-β) is a critical antiviral cytokine and is essential for innate and acquired immune responses to pathogens. Treatment with polyinosinic:polycytidylic acid (poly(I:C)) induces transient accumulation of IFN-β mRNA, which involves an increase and a decrease of IFN-β mRNA. This phenomenon has been extensively analyzed as a model for understanding the mechanisms of gene induction in response to external stimuli. Using a new RNA metabolic labeling method with ethynyluridine to directly measure de novo RNA synthesis and RNA stability, we reassessed both de novo synthesis and degradation of IFN-β mRNA. We found that transcriptional activity is maintained after the maximum accumulation of IFN-β mRNA following poly(I:C) treatment on immortalized human bronchial epithelial cells. We also observed an unexpected change in the stability of IFN-β mRNA before and after the maximum accumulation. The results indicate that this method of RNA metabolic labeling provides a general approach for the simultaneous analysis of transcriptional activity and mRNA stability coupled with transcriptional timing.
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影响因子：
7
作者：
Tani H;Mizutani R;Salam KA;Tano K;Ijiri K;Wakamatsu A;Isogai T;Suzuki Y;Akimitsu N
通讯作者：
Akimitsu N
影响因子：
4.5
作者：
Chen, Chyi-Ying A.;Yamashita, Yukiko;Shyu, Ann-Bin
通讯作者：
Shyu, Ann-Bin
影响因子：
7.3
作者：
Chen, Chyi-Ying A.;Shyu, Ann-Bin
通讯作者：
Shyu, Ann-Bin
DOI：
10.1073/pnas.0808480105
发表时间：
2008-10-14
影响因子：
11.1
作者：
Jao, Cindy Y.;Salic, Adrian
通讯作者：
Salic, Adrian
DOI：
10.1196/annals.1443.020
发表时间：
2008-01-01
期刊：
YEAR IN IMMUNOLOGY 2008
影响因子：
--
作者：
Kawai, Taro;Akira, Shizuo
通讯作者：
Akira, Shizuo