Different epitopes of the CD31 antigen identified by monoclonal antibodies: cell type-specific patterns of expression.
Different epitopes of the CD31 antigen identified by monoclonal antibodies: cell type-specific patterns of expression.
复制标题
单克隆抗体识别的 CD31 抗原的不同表位：细胞类型特异性表达模式。
作者：
L. Ashman;G. Aylett;A. Cambareri;S. Cole
3种mAb-5A2.G5、B2 B1和2BD 4-均为IgG 1同种型，通过与表达CD 31抗原的转染鼠细胞系的结合和连续免疫沉淀实验，鉴定为属于CD 31簇。使用人骨髓单核细胞系RC-2A进行竞争性结合实验。mAb B2 B1和2BD 4没有交叉阻断。mAb 5A2.G5部分抑制2BD 4的结合，但不抑制B2 B1。因此，由5A2.G5和2BD 4鉴定的表位似乎重叠，但与由B2 B1识别的表位完全分开。在血小板裂解物的非还原聚丙烯酰胺凝胶电泳后，所有3种抗体在Western印迹上结合至130 kDa物质。在衣霉素存在下培养RC-2A细胞（通过链霉蛋白酶去除表面抗原后）阻断了所有3种mAb识别的表位的再表达，表明它们涉及N-连接糖基化。此外，在电泳和Western印迹之前用糖苷内切酶F处理血小板裂解物可消除mAb与CD 31的结合，但兔多克隆抗血清不能。然而，神经氨酸酶处理RC-2A细胞未能影响mAb结合。这些抗体显示出CD 31抗体的典型特性，因为所有3种抗体均在130 kDa细胞表面蛋白处沉淀，并与血小板、单核细胞、嗜中性粒细胞和血管内皮强烈结合。所有3种抗体在CFU-C测定中产生集落的造血祖细胞上均为阳性。然而，注意到与外周血淋巴细胞和某些人白血病细胞系结合的差异。特别是，与其他2种抗体相比，mAb 5A2.G5与淋巴细胞和淋巴样细胞系HSB-2的结合较弱。
3 mAb-5A2.G5, B2B1 and 2BD4-all of IgG1 isotype were identified as belonging to the CD31 cluster by their binding to transfected murine cell lines expressing the CD31 antigen and by sequential immunoprecipitation experiments. Competitive binding experiments were carried out using the human myelomonocytic cell line RC-2A. mAb B2B1 and 2BD4 did not cross block. mAb 5A2.G5 partly inhibited binding of 2BD4 but not B2B1. Thus the epitopes identified by 5A2.G5 and 2BD4 appear to overlap but to be quite separate from that recognized by B2B1. All 3 antibodies bound to a 130 kDa species on Western blots after nonreducing polyacrylamide gel electrophoresis of platelet lysates. Culture of RC-2A cells in the presence of tunicamycin (after removal of surface antigens by pronase) blocked re-expression of the epitopes recognised by all 3 mAb suggesting that they involve N-linked glycosylation. Furthermore, treatment of platelet lysates with Endoglycosidase F prior to electrophoresis and Western blotting abolished the binding of the mAb but not a rabbit polyclonal antiserum to CD31. Nevertheless, neuraminidase treatment of RC-2A cells failed to affect mAb binding. The antibodies displayed typical properties of CD31 antibodies in that all 3 precipitated at 130 kDa cell surface protein and bound strongly to platelets, monocytes, neutrophils and vascular endothelium. All 3 antibodies were positive on hemopoietic progenitor cells which give rise to colonies in the CFU-C assay. However, differences in binding to peripheral blood lymphocytes and to certain human leukemic cell lines were noted. In particular, mAb 5A2.G5 bound weakly to lymphocytes and to the lymphoid cell line HSB-2 compared with the other 2 antibodies.
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影响因子：
56.9
作者：
CUNNINGHAM, BA;HEMPERLY, JJ;EDELMAN, GM
通讯作者：
EDELMAN, GM
影响因子：
2.2
作者：
T. V. Updyke;G. Nicolson
通讯作者：
T. V. Updyke;G. Nicolson
通过单克隆抗体 (HNK-1) 识别的人类 NK 和 K 细胞的分化抗原。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abo,T;Balch,CM
通讯作者：
Balch,CM
影响因子：
56.9
作者：
NEWMAN, PJ;BERNDT, MC;MULLER, WA
通讯作者：
MULLER, WA