Mating pair stabilization mediates bacterial conjugation species specificity.
Mating pair stabilization mediates bacterial conjugation species specificity.
复制标题
交配对稳定介导细菌接合物种特异性。
DOI：
10.1038/s41564-022-01146-4
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发表时间：
2022-07
影响因子：
28.3
通讯作者：
中科院分区：
文献类型：
--
作者：
细菌接合介导DNA从供体到受体细菌的接触转移，从而促进毒力和抗性质粒的传播。在这里，我们描述了质粒编码的供体外膜(OM)蛋白Tran的变体如何与受体中不同的OM受体合作，以介导交配对的稳定和有效的DNA转移。我们发现pKpQIL(肺炎克雷伯菌)的反式作用于OmpK36，R100-1(福氏志贺氏菌)和PSLT(鼠伤寒沙门氏菌)的质粒与OmpW相互作用，原型F质粒(大肠杆菌)与OmpA相互作用。冷冻-EM分析表明，TraNpKpQIL与OmpK36相互作用是通过在Tran末端的β-发夹插入到OmpK36孔蛋白三聚体的单体中来实现的。结合生物信息学分析和AlphaFold结构预测，我们确定了第四个反式结构变体，它通过结合OmpF来调节交配对的稳定。因此，我们根据结构相似性和与之相关的受体：反式α(OmpW)、反式β(OmpK36)、反式γ(OmpA)、反式δ(OmpF)，设计了反式同系物的分类方案。这些Tran-OM受体配对具有现实意义，因为它们反映了耐药质粒在肠杆菌科临床分离株中的分布，证明了交配对稳定在调节接合物种特异性方面的重要性。这些发现将使我们能够预测新出现的耐药质粒在高危细菌病原体中的分布。结合接合和结构分析，作者表明，反式OMP配对决定了细菌接合物种的特异性，并暗示了肠杆菌科耐药质粒的分布。
Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread of virulence and resistance plasmids. Here we describe how variants of the plasmid-encoded donor outer membrane (OM) protein TraN cooperate with distinct OM receptors in recipients to mediate mating pair stabilization and efficient DNA transfer. We show that TraN from the plasmid pKpQIL (Klebsiella pneumoniae) interacts with OmpK36, plasmids from R100-1 (Shigella flexneri) and pSLT (Salmonella Typhimurium) interact with OmpW, and the prototypical F plasmid (Escherichia coli) interacts with OmpA. Cryo-EM analysis revealed that TraNpKpQIL interacts with OmpK36 through the insertion of a β-hairpin in the tip of TraN into a monomer of the OmpK36 porin trimer. Combining bioinformatic analysis with AlphaFold structural predictions, we identified a fourth TraN structural variant that mediates mating pair stabilization by binding OmpF. Accordingly, we devised a classification scheme for TraN homologues on the basis of structural similarity and their associated receptors: TraNα (OmpW), TraNβ (OmpK36), TraNγ (OmpA), TraNδ (OmpF). These TraN-OM receptor pairings have real-world implications as they reflect the distribution of resistance plasmids within clinical Enterobacteriaceae isolates, demonstrating the importance of mating pair stabilization in mediating conjugation species specificity. These findings will allow us to predict the distribution of emerging resistance plasmids in high-risk bacterial pathogens. Combining conjugation and structural analyses, the authors show that TraN-OMP pairings determine bacterial conjugation species specificity, with implications in resistance plasmid distribution within Enterobacteriaceae.
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影响因子：
64.5
作者：
Costa TRD;Ilangovan A;Ukleja M;Redzej A;Santini JM;Smith TK;Egelman EH;Waksman G
通讯作者：
Waksman G
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1073/pnas.2003407117
发表时间：
2020-10-06
影响因子：
11.1
作者：
David S;Cohen V;Reuter S;Sheppard AE;Giani T;Parkhill J;European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group;ESCMID Study Group for Epidemiological Markers (ESGEM);Rossolini GM;Feil EJ;Grundmann H;Aanensen DM
通讯作者：
Aanensen DM
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
3.9
作者：
Argimón S;Abudahab K;Goater RJE;Fedosejev A;Bhai J;Glasner C;Feil EJ;Holden MTG;Yeats CA;Grundmann H;Spratt BG;Aanensen DM
通讯作者：
Aanensen DM