Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.
Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.
复制标题
DOI：
10.1016/j.cell.2016.08.025
复制
发表时间：
2016-09-08
期刊：
影响因子：
64.5
通讯作者：
Waksman G
中科院分区：
文献类型：
--
作者：
Costa TRD;Ilangovan A;Ukleja M;Redzej A;Santini JM;Smith TK;Egelman EH;Waksman G
接合菌毛是广泛存在的细菌附着体，在水平基因转移、抗生素耐药基因传播以及噬菌体附着等方面发挥着重要作用。在接合菌毛中，F质粒编码的F“性别”菌毛是功能最好的，也是历史上最重要的，因为F-质粒介导的接合作用的发现开启了分子生物学和遗传学的时代。然而，它的结构尚不清楚。在这里，我们介绍了两个F家族菌毛，F和pED208菌毛的原子模型，它们分别是在5.0和3.6英寸分辨率下通过低温电子显微镜重建而产生的。这些结构表明，结合菌毛是化学计量的蛋白质-磷脂单位的集合。我们进一步证明，每种菌毛类型优先与特定的磷脂结合。这些结构为F菌毛的组装提供了分子基础，也揭示了接合菌毛在细菌分泌和噬菌体感染中的显着特性。F菌毛和类F菌毛的原子结构被冷冻EM解析。该结构揭示了蛋白质-磷脂复合体单位的组装。亚单位-脂质界面的突变影响噬菌体感染和接合。该结构阐明了DNA转移和噬菌体附着的分子基础结合菌毛，使DNA能够在细菌之间转移的附件，不仅由蛋白质组成，而且，如近原子分辨低温EM所揭示的，是化学计量的蛋白质-磷脂单元的组装，磷脂成分适合于电化学促进DNA的运输。
Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F “sex” pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection. The atomic structures of the F pilus and an F-like pilus are solved by cryo-EM The structure reveals an assembly of a protein-phospholipid complex unit Mutations at subunit-lipid interfaces affect phage infection and conjugation The structure elucidates the molecular basis for DNA transfer and phage attachment Conjugative pili, the appendages that enable DNA transfer between bacteria, are not made only of protein but, as near-atomic resolution cryoEM reveals, are assemblies of stoichiometric protein-phospholipid units, with the phospholipid component suited to electrochemically facilitating DNA transport.
登录
查看更多内容
影响因子：
3.2
作者：
NOVOTNY, CP;FIVESTAY.P
通讯作者：
FIVESTAY.P
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1073/pnas.0806786105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Clarke, Margaret;Maddera, Lucinda;Silverman, Philip M.
通讯作者：
Silverman, Philip M.
影响因子：
3
作者：
Pettersen, EF;Goddard, TD;Ferrin, TE
通讯作者：
Ferrin, TE
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC