Identification of a cell-surface antigen associated with activated T lymphoblasts and activated platelets.
Identification of a cell-surface antigen associated with activated T lymphoblasts and activated platelets.
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鉴定与活化的 T 淋巴细胞和活化的血小板相关的细胞表面抗原。
DOI：
10.1182/blood.v77.1.84.bloodjournal77184
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发表时间：
1991
期刊：
影响因子：
20.3
通讯作者：
Michael A. Baker
中科院分区：
文献类型：
--
作者：
D. Sutherland;Erik Yeo;Anne Ryan;Gordon B. Mills;Dennis Bailey;Michael A. Baker
我们已经确定和生化特性的抗原，8A 3，这是活化的T淋巴母细胞和活化的血小板上表达。针对原始淋巴样/髓样细胞系KG 1a产生了针对8A 3的单克隆抗体，并且还结合到红白血病衍生的细胞系HEL，同时与一组其他造血细胞系表现出很少或没有反应性。8A 3抗原在低分化T细胞白血病和维持在白细胞介素-2（7，000位点/细胞）中的植物血凝素活化的T细胞上表达。该抗原虽然在静息血小板上未检测到，但在凝血酶活化的血小板上表达（2，000个位点/血小板）。在还原和非还原条件下，8A 3抗体在表面碘化KG 1a细胞、T淋巴母细胞和活化血小板的裂解物中鉴定出Mr为170，000和150，000的多肽。然而，肽图谱分析和与糖苷酶的同源性表明，8A 3抗原是一种Mr为170，000的单体糖蛋白，含有两个N-连接的糖苷内切酶H-敏感性聚糖，并且Mr为150，000的结构是通过蛋白水解降解而衍生的。8A 3抗原在体内KG 1a细胞中没有可检测的磷酸化，含有它的免疫复合物在体外也没有表现出激酶活性。8A 3抗原的结构和血清学特征表明，它不同于先前描述的其它白细胞活化抗原，包括转铁蛋白受体、白细胞介素-2受体、粘附分子的整联蛋白家族成员或白细胞共同抗原/CD 45簇的“限制性”成员。此外，8A 3抗原似乎与先前描述的其他活化特异性血小板分子GMP 140/PADGEM无关。该抗体可用于监测某些临床情况下的T细胞活化状态，并表征临床相关的活化相关血小板膜改变。
We have identified and biochemically characterized an antigen, 8A3, which is expressed on activated T lymphoblasts and activated platelets. Monoclonal antibodies to 8A3 were raised against the primitive lymphoid/myeloid cell line KG1a and additionally bound to the erythroleukemia-derived cell line HEL, whilst exhibiting little or no reactivity with a panel of other hematopoietic cell lines. The 8A3 antigen was expressed on poorly differentiated T-cell leukemias and on phytohemagglutinin-activated T-cells maintained in interleukin-2 (7,000 sites/cell). This antigen, though not detected on resting platelets, was expressed on thrombin-activated platelets (2,000 sites/platelet). Antibodies to 8A3 identified polypeptides of Mr 170,000 and 150,000 in lysates of surface-iodinated KG1a cells, T lymphoblasts, and activated platelets under both reducing and nonreducing conditions. However, peptide mapping and susceptibily to glycosidases indicated that the 8A3 antigen was a monomeric glycoprotein of Mr 170,000 which contained two N-linked endoglycosidase H-sensitive glycans, and that the Mr 150,000 structure was derived from it by proteolytic degradation. The 8A3 antigen was not detectably phosphorylated in KG1a cells in vivo, nor did immune complexes containing it exhibit kinase activity in vitro. Structural and serologic characteristics of the 8A3 antigen indicate that it is different from other previously described leukocyte activation antigens including transferrin receptors, interleukin-2 receptors, members of the integrin family of adhesion molecules, or "restricted" members of the leukocyte-common antigen/CD45 cluster. Furthermore, the 8A3 antigen does not appear to be related to the other previously described activation-specific platelet molecule, GMP140/PADGEM. This antibody may be useful in monitoring T-cell activation status in some clinical situations and in characterizing clinically relevant activation-associated platelet membrane alterations.
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单克隆抗体 (A-1A5) 识别的新型分化抗原复合物的表征：受刺激的 T 细胞上独特的激活特异性分子形式。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hemler,ME;Ware,CF;Strominger,JL
通讯作者：
Strominger,JL
影响因子：
56.9
作者：
MARTIN, P;PAPAYANNOPOULOU, T
通讯作者：
PAPAYANNOPOULOU, T
DOI：
10.1016/s0021-9258(17)47274-7
发表时间：
1984-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Hsu-Lin;C. L. Berman;B. Furie;D. August;B. Furie
通讯作者：
S. Hsu-Lin;C. L. Berman;B. Furie;D. August;B. Furie
DOI：
--
发表时间：
1984-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino
通讯作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino
对人类细胞杂种中肿瘤相关膜磷蛋白具有特异性的单克隆抗体。
影响因子：
--
作者：
Bicknell,DC;Sutherland,DR;Stanbridge,EJ;Greaves,MF
通讯作者：
Greaves,MF