Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
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DOI：
10.1371/journal.pone.0082096
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bosio CM
中科院分区：
文献类型：
--
作者：
Griffin AJ;Crane DD;Wehrly TD;Scott DP;Bosio CM
图拉氏方济氏菌是一种细胞内的革兰氏阴性细菌，在吸入不到15种微生物后会导致急性致死性疾病。方济各氏菌感染的致病性与其逃避和抑制宿主细胞炎症反应的独特能力有关。已经提出，诱导感染的巨噬细胞的交替激活是减毒方济氏菌调节宿主反应的一种机制。在这份报告中，我们发现无论是减毒的图拉氏杆菌活疫苗株(LVS)还是图拉氏杆菌强毒株SchuS4，在体内外都不能诱导巨噬细胞的交替激活。LVS，而不是SchuS4，在体外和体内刺激精氨酸酶1的产生，而不是依赖于交替激活。然而，精氨酸酶1的缺失并不显著影响LVS或SchuS4的细胞内复制。总之，我们的数据表明，诱导交替激活或表达精氨酸酶1都不是由弱毒力弗朗西斯氏菌介导的疾病的关键特征。
Virulent Francisella tularensis ssp tularensis is an intracellular, Gram negative bacterium that causes acute lethal disease following inhalation of fewer than 15 organisms. Pathogenicity of Francisella infections is tied to its unique ability to evade and suppress inflammatory responses in host cells. It has been proposed that induction of alternative activation of infected macrophages is a mechanism by which attenuated Francisella species modulate host responses. In this report we reveal that neither attenuated F. tularensis Live Vaccine Strain (LVS) nor virulent F. tularensis strain SchuS4 induce alternative activation of macrophages in vitro or in vivo. LVS, but not SchuS4, provoked production of arginase1 independent of alternative activation in vitro and in vivo. However, absence of arginase1 did not significantly impact intracellular replication of LVS or SchuS4. Together our data establish that neither induction of alternative activation nor expression of arginase1 are critical features of disease mediated by attenuated or virulent Francisella species.
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影响因子：
30.3
作者：
Qualls JE;Subramanian C;Rafi W;Smith AM;Balouzian L;DeFreitas AA;Shirey KA;Reutterer B;Kernbauer E;Stockinger S;Decker T;Miyairi I;Vogel SN;Salgame P;Rock CO;Murray PJ
通讯作者：
Murray PJ
影响因子：
5.4
作者：
Barron, Luke;Wynn, Thomas A.
通讯作者：
Wynn, Thomas A.
影响因子：
8
作者：
通讯作者：
--
影响因子：
3.1
作者：
Chase, Jennifer C.;Celli, Jean;Bosio, Catharine M.
通讯作者：
Bosio, Catharine M.
DOI：
10.1073/pnas.0707636105
发表时间：
2008-07-08
影响因子：
11.1
作者：
Ketavarapu, Jyothi M.;Rodriguez, Annette R.;Arulanandam, Bernard P.
通讯作者：
Arulanandam, Bernard P.