DNA Dendrons as Agents for Intracellular Delivery.
DNA Dendrons as Agents for Intracellular Delivery.
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DOI：
10.1021/jacs.1c07240
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发表时间：
2021-09-01
影响因子：
15
通讯作者：
Mirkin CA
中科院分区：
文献类型：
--
作者：
Distler ME;Teplensky MH;Bujold KE;Kusmierz CD;Evangelopoulos M;Mirkin CA
Herein, a method for synthesizing and utilizing DNA dendrons to deliver biomolecules to living cells is reported. Inspired by high-density nucleic acid nanostructures, such as spherical nucleic acids, we hypothesized that small clusters of nucleic acids, in the form of DNA dendrons, could be conjugated to biomolecules and facilitate their cellular uptake. We show that DNA dendrons are internalized by 90% of dendritic cells after just 1 h of treatment, with a >20-fold increase in DNA delivery per cell compared with their linear counterparts. This effect is due to the interaction of the DNA dendrons with scavenger receptor-A on cell surfaces, which results in their rapid endocytosis. Moreover, when conjugated to peptides at a single attachment site, dendrons enhance the cellular delivery and activity of both the model ovalbumin 1 peptide and the therapeutically relevant thymosin alpha 1 peptide. These findings show that high-density, multivalent DNA ligands play a significant role in dictating cellular uptake of biomolecules and consequently will expand the scope of deliverable biomolecules to cells. Indeed, DNA dendrons are poised to become agents for the cellular delivery of many molecular and nanoscale materials.
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影响因子：
15
作者：
Banga RJ;Meckes B;Narayan SP;Sprangers AJ;Nguyen ST;Mirkin CA
通讯作者：
Mirkin CA
影响因子：
15
作者：
Brodin JD;Sprangers AJ;McMillan JR;Mirkin CA
通讯作者：
Mirkin CA
影响因子：
18.2
作者：
Kusmierz, Caroline D.;Bujold, Katherine E.;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.
影响因子：
4.2
作者：
Bruno BJ;Miller GD;Lim CS
通讯作者：
Lim CS
影响因子：
5.6
作者：
Kristensen M;Birch D;Mørck Nielsen H
通讯作者：
Mørck Nielsen H