Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus
Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus
复制标题
甘蔗花叶病毒玉米抗感基因型的磷酸化蛋白质组学分析
DOI：
10.1007/s00726-014-1880-2
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发表时间：
2014-12
期刊：
影响因子：
3.5
通讯作者：
Xiuli Hu
中科院分区：
文献类型：
--
作者：
Liuji Wu;Shunxi Wang;Jianyu Wu;Zanping Han;Rui Wang;Liancheng Wu;Huimin Zhang;Yanhui Chen;Xiuli Hu
蛋白质磷酸化在许多细胞事件的调节中起着关键作用。然而，还没有关于植物对病毒感染的蛋白质磷酸化的信息。在这项研究中，我们的特点是磷蛋白质组的抗和感基因型玉米（玉米L。）甘蔗花叶病毒（SCMV）感染。基于同位素标记相对和绝对定量技术、TiO 2富集法和LC-MS/MS分析，我们分别鉴定了65个和59个磷蛋白，其磷酸化水平在感病和抗病植物中受到显著调节。一些鉴定的磷蛋白由两种基因型共享，表明对病毒感染的反应途径部分重叠。虽然几种磷蛋白是众所周知的病原体反应磷蛋白，但病毒感染差异调节大多数其他磷蛋白，这在文献中尚未报道。蛋白磷酸化状态的变化表明，对SCMV感染的反应包括主要细胞过程的重新格式化。我们的数据为植物-病毒相互作用提供了新的有价值的见解。
Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to virus infection. In this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (Zea mays L.) in response to Sugarcane mosaic virus (SCMV) infection. Based on isotope tags for relative and absolute quantification technology, TiO2 enrichment method and LC-MS/MS analysis, we identified 65 and 59 phosphoproteins respectively, whose phosphorylation level regulated significantly in susceptible and resistant plants. Some identified phosphoproteins were shared by both genotypes, suggesting a partial overlapping of the responsive pathways to virus infection. While several phosphoproteins are well-known pathogen response phosphoproteins, virus infection differentially regulates most other phosphoproteins, which has not been reported in literature. Changes in protein phosphorylation status indicated that response to SCMV infection encompass a reformatting of major cellular processes. Our data provide new valuable insights into plant-virus interactions.
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影响因子：
6.2
作者：
Gan, Defang;Zhang, Jiao;Cheng, Beijiu
通讯作者：
Cheng, Beijiu
影响因子：
3.2
作者：
Perera, M. F.;Filippone, M. P.;Castagnaro, A. P.
通讯作者：
Castagnaro, A. P.
影响因子：
3.3
作者：
Huang, Chao;Verrillo, Francesca;Marra, Mauro
通讯作者：
Marra, Mauro
DOI：
10.1073/pnas.0610208104
发表时间：
2007-04
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Ryoung Shin;S. Alvarez;Adrien Y. Burch;J. Jez;D. Schachtman
通讯作者：
Ryoung Shin;S. Alvarez;Adrien Y. Burch;J. Jez;D. Schachtman
影响因子：
7
作者：
Larsen, MR;Thingholm, TE;Jorgensen, TJD
通讯作者：
Jorgensen, TJD