Full-length transcriptome sequences and the identification of putative genes for flavonoid biosynthesis in safflower.
Full-length transcriptome sequences and the identification of putative genes for flavonoid biosynthesis in safflower.
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红花类黄酮生物合成的全长转录组序列和推定基因的鉴定
DOI：
10.1186/s12864-018-4946-9
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发表时间：
2018-07-24
期刊：
影响因子：
4.4
通讯作者：
Pei J
中科院分区：
文献类型：
--
作者：
Chen J;Tang X;Ren C;Wei B;Wu Y;Wu Q;Pei J
背景红花(Carthamus tinctorius L.)已被广泛应用于中医，具有改善脑血流的能力。黄酮类化合物是红花中的主要生物活性成分，其生物合成引起了人们的广泛兴趣。以前的研究大多使用第二代测序平台来调查可能的类黄酮生物合成基因。为了更好地了解红花的转录数据和参与红花类黄酮生物合成的可能基因，我们进行了研究。结果从6种红花组织中提取到高质量的RNA。不同组织的RNA均匀混合，用于多个大小不同的文库(1-2，2-3和3-6个)文库的构建。携带5个细胞(2个细胞用于1-2和2-3个k文库，1个细胞用于3-6个k文库)。捕获了10.43 Gb的干净数据和38,302个去冗余序列。44个独特的异构体被注释为参与类黄酮生物合成的编码酶。对全长类黄酮基因进行了特征分析，分析了它们的进化关系和表达模式。它们可以分为8个家族，在组织表达上有很大的差异。在MeJA处理下，有9个基因表达显著上调，2个基因表达显著下调。在本研究中，我们预测了参与红花类黄酮合成的基因。此外，我们还对SSR和lncRNA进行了分析。结论本研究使用了全长转录组序列。在本研究中，我们预测了参与红花类黄酮合成的基因。结合总黄酮的测定，CtC4H2、CtCHS3、CtCHI3、CtF3H3、CtF3H1主要参与MeJA促进总黄酮的合成。我们的研究结果也为红花的进一步研究提供了有价值的资源。
BackgroundThe flower of the safflower (Carthamus tinctoriusL.) has been widely used in traditional Chinese medicine for the ability to improve cerebral blood flow. Flavonoids are the primary bioactive components in safflower, and their biosynthesis has attracted widespread interest. Previous studies mostly used second-generation sequencing platforms to survey the putative flavonoid biosynthesis genes. For a better understanding of transcription data and the putative genes involved in flavonoid biosynthesis in safflower, we carry our study.ResultsHigh-quality RNA was extracted from six types of safflower tissue. The RNAs of different tissues were mixed equally and used for multiple size-fractionated libraries (1–2, 2–3 and 3-6 k) library construction. Five cells were carried (2 cells for 1–2 and for 2-3 k libraries and 1 cell for 3-6 k libraries). 10.43Gb clean data and 38,302 de-redundant sequences were captured. 44 unique isoforms were annotated as encoding enzymes involved in flavonoid biosynthesis. The full length flavonoid genes were characterized and their evolutional relationship and expressional pattern were analyzed. They can be divided into eight families, with a large differences in the tissue expression. The temporal expressions under MeJA treatment were also measured, 9 genes are significantly up-regulated and 2 genes are significantly down-regulated. The genes involved in flavonoid synthesis in safflower were predicted in our study. Besides, the SSR and lncRNA are also analyzed in our study.ConclusionsFull-length transcriptome sequences were used in our study. The genes involved in flavonoid synthesis in safflower were predicted in our study. Combined the determination of flavonoids,CtC4H2,CtCHS3,CtCHI3,CtF3H3,CtF3H1are mainly participated in MeJA promoting the synthesis of flavonoids. Our results also provide a valuable resource for further study on safflower.
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不同化学型红花品系和 MeJA 刺激下三个 UGT 基因的表达模式揭示了它们在类黄酮生物合成中的潜在作用
影响因子：
3.7
作者：
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作者：
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影响因子：
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作者：
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