The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis.
The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis.
复制标题
AtMDN1 氨基酸 3838 处的 Glu 突变引发拟南芥多效性发育表型
DOI：
10.1038/srep36446
复制
发表时间：
2016-11-08
影响因子：
4.6
通讯作者：
Zheng CC
中科院分区：
文献类型：
--
作者：
Li PC;Yu SW;Li K;Huang JG;Wang XJ;Zheng CC
MDN 1/Rea 1是一种AAA型ATP酶，被认为是大多数生物中参与前核糖体成熟的最大蛋白。然而，它在植物生长和发育中的作用却知之甚少。在这里，我们的特点是一种新的拟南芥突变体，矮生短根（DSR）1，它显示出多效性的发展表型，如发芽缓慢，短根，矮生芽，并减少在正常生长条件下的种子集。使用定位克隆，我们发现，AtMDN 1功能受损的“谷氨酸”到“赖氨酸”的变化在位置3838的氨基酸序列在dsr 1。多序列比对分析表明，AtMDN 1的连接结构域中的Glu残基在生物体中高度保守。AtMDN 1在多种组织中表达，特别是在茎尖和根尖中。此外，转录谱分析结果表明，dsr 1中AtMDN 1的功能障碍会影响植物生长发育相关基因的表达，这与dsr 1的多效性表型密切相关。因此，我们得出结论，Glu残基在维持AtMDN 1功能中起着至关重要的作用，这对植物生长和发育至关重要。
MDN1/Rea1, as an AAA-type ATPase, is predicted to be the largest protein involved in pre-ribosome maturation in most organisms. However, its function in plant growth and development is poorly understood. Here, we characterized a novel Arabidopsis mutant, dwarf & short root (dsr) 1, which shows pleiotropic developmental phenotypes, such as slow germination, short root, dwarf shoot, and reduced seed set under normal growth conditions. Using positional cloning, we revealed that the AtMDN1 function is impaired by a ‘glutamic acid’ to ‘lysine’ change at position 3838 of the amino acid sequence in dsr1. Multiple sequence alignment analysis revealed that the mutated Glu residue, which located in the linker domain of AtMDN1, is extremely conserved among organisms. AtMDN1 is expressed in various tissues, particularly in the shoot apex and root tip. Moreover, the results of transcript profile analyses showed that the dysfunction of AtMDN1 in dsr1 impairs the expression of genes related to plant growth and development, which is tightly associated with the pleiotropic phenotypes of dsr1. Thus, we concluded that the Glu residue plays a vital role in maintaining AtMDN1 functions, which are essential for plant growth and development.
登录
查看更多内容
影响因子：
7.8
作者：
Bassler, Jochen;Paternoga, Helge;Hurt, Ed
通讯作者：
Hurt, Ed
影响因子：
4.8
作者：
Galani, K;Nissan, TA;Hurt, E
通讯作者：
Hurt, E
影响因子：
16.8
作者：
Barrio-Garcia, Clara;Thoms, Matthias;Hurt, Ed
通讯作者：
Hurt, Ed
DOI：
10.1016/j.bbamcr.2011.06.017
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Kressler D;Hurt E;Bergler H;Bassler J
通讯作者：
Bassler J
影响因子：
16
作者：
Joly, Nicolas;Zhang, Nan;Buck, Martin
通讯作者：
Buck, Martin