Protective effect of pomegranate-derived products on UVB-mediated damage in human reconstituted skin.
Protective effect of pomegranate-derived products on UVB-mediated damage in human reconstituted skin.
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DOI：
10.1111/j.1600-0625.2008.00829.x
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发表时间：
2009-06
影响因子：
3.6
通讯作者：
Mukhtar H
中科院分区：
文献类型：
--
作者：
Afaq F;Zaid MA;Khan N;Dreher M;Mukhtar H
太阳紫外线(UV)辐射，特别是其UVB(290-320 nm)成分，是许多不利生物效应的主要原因，包括光老化和皮肤癌。UVB辐射可引起DNA损伤、蛋白质氧化和诱导基质金属蛋白酶(MMPs)。通过使用植物抗氧化剂进行光化学预防，为人类皮肤提供保护，使其免受UVB的损害，正受到越来越多的关注。从石榴树中提取的石榴，含有花青素和水解单宁，具有很强的抗氧化和抗肿瘤作用。在这项研究中，我们在重组人皮肤(Epiderm™FT-200)上测定了石榴衍生产品POMx汁、POMx提取物和石榴油(POMO)对中波紫外线诱导的损伤的保护作用。分别用POMx汁(1~2μL/0.1ml/孔)、POMx提取液(5~10μg/0.1ml/孔)、POMO(1~2μL/0.1ml/孔)处理表皮1h，12h后取材，用免疫印迹和免疫组织化学方法检测蛋白质氧化、DNA损伤标志物和光老化情况。石榴提取物可抑制UVB诱导的环丁烷嘧啶二聚体、8-二氢-2‘-脱氧鸟苷、蛋白质氧化和增殖细胞核抗原的表达。石榴提取物可抑制UVB诱导的胶原酶(MMP1)、明胶酶(MMP2、MMP9)、基质分解酶(MMP3)、大蒜素(MMP7)、弹性蛋白酶(MMP12)和原弹性蛋白。明胶酶谱显示石榴提取物可抑制UVB诱导的基质金属蛋白酶-2和基质金属蛋白酶-9的活性。石榴提取物还能降低UVB诱导的c-Fos蛋白表达和c-jun的磷酸化。总而言之，这些结果表明，所有三种石榴衍生产品都可能有助于对抗UVB对人体皮肤的损害。
Solar ultraviolet (UV) radiation, particularly its UVB (290-320 nm) component, is the primary cause of many adverse biological effects including photoaging and skin cancer. UVB radiation causes DNA damage, protein oxidation and induces matrix metalloproteinases (MMPs). Photochemoprevention via the use of botanical antioxidants in affording protection to human skin against UVB damage is receiving increasing attention. Pomegranate, from the tree Punica granatum contains anthocyanins and hydrolyzable tannins and possesses strong anti-oxidant and anti-tumor promoting properties. In this study, we determined the effect of pomegranate derived products POMx juice, POMx extract and pomegranate oil (POMo) against UVB-mediated damage using reconstituted human skin (EpiDerm™ FT-200). EpiDerm was treated with POMx juice (1-2 μl/0.1 ml/well), POMx extract (5-10 μg/0.1 ml/well), and POMo (1-2 μl/0.1 ml/well) for 1 h prior to UVB (60 mJ/cm2) irradiation and was harvested 12 h post-UVB to assess protein oxidation, markers of DNA damage and photoaging by western blot analysis and immunohistochemistry. Pretreatment of Epiderm with pomegranate derived products resulted in inhibition of UVB-induced (i) cyclobutane pyrimidine dimers, (ii) 8-dihydro-2′-deoxyguanosine, (iii) protein oxidation, and (iv) PCNA protein expression. We also found that pretreatment of Epiderm with pomegranate derived products resulted in inhibition of UVB-induced (i) collagenase (MMP-1), (ii) gelatinase (MMP-2, MMP-9), (iii) stromelysin (MMP-3), (iv) marilysin (MMP-7), (v) elastase (MMP-12), and (vi) tropoelastin. Gelatin zymography revealed that pomegranate derived products inhibited UVB-induced MMP-2 and MMP-9 activities. Pomegranate derived products also caused a decrease in UVB-induced protein expression of c-Fos and phosphorylation of c-Jun. Collectively, these results suggest that all three pomegranate derived products may be useful against UVB-induced damage to human skin.
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影响因子：
6.4
作者：
Afaq, F;Saleem, M;Mukhtar, H
通讯作者：
Mukhtar, H
影响因子：
11.2
作者：
Khan, Naghma;Afaq, Farrukh;Mukhtar, Hasan
通讯作者：
Mukhtar, Hasan
影响因子：
6.5
作者：
Chung, JH;Seo, JY;Eun, HC
通讯作者：
Eun, HC
影响因子：
4.8
作者：
Böhm, M;Wolff, I;Schwarz, A
通讯作者：
Schwarz, A
DOI：
10.1073/pnas.93.10.4827
发表时间：
1996-05-14
影响因子：
11.1
作者：
Huang, LC;Clarkin, KC;Wahl, GM
通讯作者：
Wahl, GM