Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology.
Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology.
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亵渎半硫酸盐作为护理点细胞学的荧光造影剂。
DOI：
10.1371/journal.pone.0125598
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Muldoon TJ
中科院分区：
文献类型：
--
作者：
Prieto SP;Powless AJ;Boice JW;Sharma SG;Muldoon TJ
半硫酸脯黄素是一种吖啶衍生的荧光染料，可用于生物标本的细胞学检查。由于其小的两亲性结构和嵌入DNA的能力，核黄素荧光染色细胞核和细胞质结构。在这份手稿中，我们证明了使用原黄素作为一个快速的细胞学染料对一些标本，包括正常脱落的口腔鳞状细胞，培养的人口腔鳞状细胞癌细胞，白细胞来自全血标本，使用定制的，便携式，LED照明的荧光显微镜。在将细胞悬浮于稀释于盐水中的0.01%（w/v）原黄素中后，不需要孵育时间。原黄素染色的口腔细胞的图像具有清晰可见的细胞核以及颗粒状细胞质，而染色的白细胞显示明亮的细胞核，并突出了中性粒细胞中细胞核的多叶性。我们还证明了原黄素染色细胞的数字图像的定量分析的实用性，其可用于检测不同细胞类型之间的显著形态学差异。原黄素染色的口腔细胞具有明确的细胞核和细胞膜，其允许定量分析细胞核与细胞质的比率，以及图像纹理分析以提取定量图像特征。
Proflavine hemisulfate, an acridine-derived fluorescent dye, can be used as a rapid stain for cytologic examination of biological specimens. Proflavine fluorescently stains cell nuclei and cytoplasmic structures, owing to its small amphipathic structure and ability to intercalate DNA. In this manuscript, we demonstrated the use of proflavine as a rapid cytologic dye on a number of specimens, including normal exfoliated oral squamous cells, cultured human oral squamous carcinoma cells, and leukocytes derived from whole blood specimens using a custom-built, portable, LED-illuminated fluorescence microscope. No incubation time was needed after suspending cells in 0.01% (w/v) proflavine diluted in saline. Images of proflavine stained oral cells had clearly visible nuclei as well as granular cytoplasm, while stained leukocytes exhibited bright nuclei, and highlighted the multilobar nature of nuclei in neutrophils. We also demonstrated the utility of quantitative analysis of digital images of proflavine stained cells, which can be used to detect significant morphological differences between different cell types. Proflavine stained oral cells have well-defined nuclei and cell membranes which allowed for quantitative analysis of nuclear to cytoplasmic ratios, as well as image texture analysis to extract quantitative image features.
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影响因子：
3.8
作者：
KUBOTA, Y;STEINER, RF
通讯作者：
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影响因子：
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作者：
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