Genome-Wide Analysis of the RNase T2 Family and Identification of Interacting Proteins of Four ClS-RNase Genes in 'XiangShui' Lemon.
Genome-Wide Analysis of the RNase T2 Family and Identification of Interacting Proteins of Four ClS-RNase Genes in 'XiangShui' Lemon.
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DOI：
10.3390/ijms231810431
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发表时间：
2022-09-09
影响因子：
5.6
通讯作者：
He, Xin-Hua
中科院分区：
文献类型：
--
作者：
Li, Yu-Ze;Zhu, Jia-Wei;Lin, Wei;Lan, Mo-Ying;Luo, Cong;Xia, Li-Ming;Zhang, Yi-Li;Liang, Rong-Zhen;Hu, Wang-Li;Huang, Gui-Xiang;He, Xin-Hua
S核糖核酸酶在芸香科植物自交不亲和过程中起重要作用。资料表明，自花授粉过程中的排斥现象是由于S核糖核酸酶降解花粉管核糖核酸所致。花粉管的细胞骨架微丝被破坏，其他成分无法从柱头向下延伸，最终无法到达子房完成受精。本研究从响水柠檬基因组和泛素组中鉴定了4个S核糖核酸酶基因序列。序列分析表明，‘响水’柠檬S核糖核酸酶中保守的核糖核酸酶T2结构域与其他物种的相同。表达模式分析表明，S3-RNase和S4-RNase在雌蕊中特异表达，时空表达分析表明，自花授粉后柱头、花柱和子房中S3-RNase的表达水平显著高于异花授粉后。激光共聚焦显微镜下的亚细胞定位分析表明，S1-RNase、S2-RNase、S3-RNase和S4-RNase在细胞核内均有表达。此外，酵母双杂交(Y2H)分析表明，S3-RNase与F-box、双功能岩藻糖/岩藻糖焦磷酸化酶(FKGP)、天冬氨酸蛋白酶A1、RRP46、果胶酶/果胶酯酶抑制因子51(PME51)、磷脂：二酰甘油酰基转移酶1(PDAT1)、赤霉素受体GID1B、GDT1样蛋白4、可能的转化酶抑制物、tRNA连接酶、PAP15、PAE8、TIM14-2、PGIP1和p24beta2相互作用。此外，S3-核糖核酸酶与TOPP4相互作用。因此，S3-核糖核酸酶可能在响水柠檬的SI中起重要作用。
S-RNase plays vital roles in the process of self-incompatibility (SI) in Rutaceae plants. Data have shown that the rejection phenomenon during self-pollination is due to the degradation of pollen tube RNA by S-RNase. The cytoskeleton microfilaments of pollen tubes are destroyed, and other components cannot extend downwards from the stigma and, ultimately, cannot reach the ovary to complete fertilisation. In this study, four S-RNase gene sequences were identified from the ‘XiangShui’ lemon genome and ubiquitome. Sequence analysis revealed that the conserved RNase T2 domains within S-RNases in ‘XiangShui’ lemon are the same as those within other species. Expression pattern analysis revealed that S3-RNase and S4-RNase are specifically expressed in the pistils, and spatiotemporal expression analysis showed that the S3-RNase expression levels in the stigmas, styles and ovaries were significantly higher after self-pollination than after cross-pollination. Subcellular localisation analysis showed that the S1-RNase, S2-RNase, S3-RNase and S4-RNase were found to be expressed in the nucleus according to laser confocal microscopy. In addition, yeast two-hybrid (Y2H) assays showed that S3-RNase interacted with F-box, Bifunctional fucokinase/fucose pyrophosphorylase (FKGP), aspartic proteinase A1, RRP46, pectinesterase/pectinesterase inhibitor 51 (PME51), phospholipid:diacylglycerol acyltransferase 1 (PDAT1), gibberellin receptor GID1B, GDT1-like protein 4, putative invertase inhibitor, tRNA ligase, PAP15, PAE8, TIM14-2, PGIP1 and p24beta2. Moreover, S3-RNase interacted with TOPP4. Therefore, S3-RNase may play an important role in the SI of ‘XiangShui’ lemon.
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影响因子：
11.4
作者：
Mokranjac, Dejana;Bourenkov, Gleb;Groll, Michael
通讯作者：
Groll, Michael
IBS：用于生物序列呈现和可视化的插画家
DOI：
10.1093/bioinformatics/btv362
发表时间：
2015-10-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Liu W;Xie Y;Ma J;Luo X;Nie P;Zuo Z;Lahrmann U;Zhao Q;Zheng Y;Zhao Y;Xue Y;Ren J
通讯作者：
Ren J
影响因子：
64.8
作者：
Thomas, SG;Franklin-Tong, VE
通讯作者：
Franklin-Tong, VE
影响因子：
64.8
作者：
ANDERSON, MA;CORNISH, EC;CLARKE, AE
通讯作者：
CLARKE, AE
影响因子：
7.4
作者：
Kuang, Ruibin;Chan, Kam-Ho;Lim, Boon Leong
通讯作者：
Lim, Boon Leong