Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.
Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.
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DOI：
10.1038/s41598-018-30360-6
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发表时间：
2018-08-10
影响因子：
4.6
通讯作者：
Prodromakis T
中科院分区：
文献类型：
--
作者：
Sanzari I;Humphrey EJ;Dinelli F;Terracciano CM;Prodromakis T
我们最近证明，图案化的聚对二甲苯C膜可以有效地用作掩模，用于在聚丙烯酰胺水凝胶（PAm）上直接共聚蛋白质。在这项工作中，我们已经证明了这种技术的适用性，用于研究这种平台对新生大鼠心室肌细胞（NRVMs）的影响。首先，我们在纳米级水平上对液体中的支架进行了形貌和机械表征。因此，我们确定这样的平台具有紧密模拟细胞的体内细胞外环境的物理性质。然后，我们研究了细胞的形态和生理学通过比较培养平坦均匀覆盖和胶原图案的支架。我们发现，微图案促进细胞沿着胶原蛋白包被的脊的主轴伸长。在某些情况下，细胞也倾向于在凹槽上形成桥梁。我们最终研究了细胞收缩，在一定的刺激下监测Ca2+循环。与各向同性支架相比，接种在图案化支架上的细胞呈现显著的响应。
We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the applicability of this technique for studying the effect such platforms render on neonatal rat ventricular myocytes (NRVMs). Firstly, we have characterised topographically and mechanically the scaffolds in liquid at the nano-scale level. We thus establish that such platforms have physical properties that closely mimics the in vivo extracellular environment of cells. We have then studied the cell morphology and physiology by comparing cultures on flat uniformly-covered and collagen-patterned scaffolds. We show that micro-patterns promote the elongation of cells along the principal axis of the ridges coated with collagen. In several cases, cells also tend to create bridges across the grooves. We have finally studied cell contraction, monitoring Ca2+ cycling at a certain stimulation. Cells seeded on patterned scaffolds present significant responses in comparison to the isotropic ones.
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影响因子：
3.5
作者：
Di Benedetto, F;Biasco, A;Cingolani, R
通讯作者：
Cingolani, R
影响因子：
14
作者：
Rao, Christopher;Prodromakis, Themistoklis;Kolker, Ljudmila;Chaudhry, Umar A. R.;Trantidou, Tatiana;Sridhar, Arun;Weekes, Claire;Camelliti, Patrizia;Harding, Sian E.;Darzi, Ara;Yacoub, Magdi H.;Athanasiou, Thanos;Terracciano, Cesare M.
通讯作者：
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影响因子：
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作者：
Au, Hoi Ting Heidi;Cui, Bo;Radisic, Milica
通讯作者：
Radisic, Milica
影响因子：
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作者：
Denisin, Aleksandra K.;Pruitt, Beth L.
通讯作者：
Pruitt, Beth L.
影响因子：
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作者：
Bhana, Bashir;Iyer, Rohin K.;Radisic, Milica
通讯作者：
Radisic, Milica