Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
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DOI：
10.1371/journal.pone.0032052
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Okabe H
中科院分区：
文献类型：
--
作者：
Hama Y;Chano T;Inui T;Matsumoto K;Okabe H
Rb1诱导的卷曲线圈1(RB1CC1；又称FIP200)在细胞增殖和自噬等多种生物学途径中发挥重要作用。评估RB1CC1的表达可以为各种癌症和神经退行性疾病提供有用的临床信息。为了实现临床应用，有必要建立稳定的抗体供应和可重复性的实验室检测程序。在本研究中，我们制备了针对RB1CC1的小鼠单抗，并发现四种抗体(N1-8、N1-216、N3-2和N3-42)最适合于临床应用，如EL ISA、免疫印迹和Work以及已有的多克隆抗体。在福尔马林固定石蜡包埋组织的临床病理检查中，N1-8单抗对RB1CC1的识别效果最好。这些单抗将有助于在生物学和临床医学的科学检查中创造新的机会。
RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.
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