Low-dose vasopressin infusion results in increased mortality and cardiac dysfunction following ischemia-reperfusion injury in mice.
Low-dose vasopressin infusion results in increased mortality and cardiac dysfunction following ischemia-reperfusion injury in mice.
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DOI：
10.1186/cc7930
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Walley KR
中科院分区：
文献类型：
--
作者：
Indrambarya T;Boyd JH;Wang Y;McConechy M;Walley KR
精氨酸加压素是一种血管活性药物，通常用于分布性休克状态，包括带有心脏成分的混合性休克。然而，精氨酸加压素对缺血/再灌注损伤心脏功能的直接影响尚不清楚。我们用经胸超声心动图测量了C57B6小鼠的左心室射血分数，包括正常对照组和结扎左冠状动脉前降支1小时所致的缺血/再灌注损伤。给小鼠注射生理盐水、多巴酚丁胺(8.33U/kg/μ)或精氨酸加压素(0.00057单位/kg/分钟，相当于每70公斤人体0.04U/分钟)。使用微压计导管测量动脉血压。此外，还记录了死亡率，并对心脏组织进行了RNA和蛋白质处理。基线左心室射血分数为65.6%(60~72)。在正常对照小鼠中，不同输液组的左心室射血分数没有差异。在缺血/再灌注损伤后，AVP治疗第1天的左心室射血分数显著降低46.2%(34.4至52.0)，与基线和生理盐水治疗的第1天对照组相比均降低56.9%(42.4至60.2)。AVP对左心室射血分数的影响在前负荷(左心室舒张末容量)、后负荷(血压)或心率方面没有显著差异。精氨酸加压素组的7天死亡率最高。在缺血/再灌注损伤后，我们发现心脏V1受体的表达没有变化，但催产素受体的表达下降了40%。与生理盐水和多巴酚丁胺治疗的动物相比，精氨酸加压素输注显著抑制了缺血/再灌注模型的心肌功能，并增加了死亡率。在与严重心脏损伤相关的非血管扩张性休克状态下，可能禁忌使用加压素。
Arginine vasopressin is a vasoactive drug commonly used in distributive shock states including mixed shock with a cardiac component. However, the direct effect of arginine vasopressin on the function of the ischemia/reperfusion injured heart has not been clearly elucidated. We measured left ventricular ejection fraction using trans-thoracic echocardiography in C57B6 mice, both in normal controls and following ischemia/reperfusion injury induced by a one hour ligation of the left anterior descending coronary artery. Mice were treated with one of normal saline, dobutamine (8.33 μg/kg/min), or arginine vasopressin (0.00057 Units/kg/min, equivalent to 0.04 Units/min in a 70 kg human) delivered by an intraperitoneal micro-osmotic pump. Arterial blood pressure was measured using a micromanometer catheter. In addition, mortality was recorded and cardiac tissues processed for RNA and protein. Baseline left ventricular ejection fraction was 65.6% (60 to 72). In normal control mice, there was no difference in left ventricular ejection fraction according to infusion group. Following ischemia/reperfusion injury, AVP treatment significantly reduced day 1 left ventricular ejection fraction 46.2% (34.4 to 52.0), both in comparison with baseline and day 1 saline treated controls 56.9% (42.4 to 60.2). There were no significant differences in preload (left ventricular end diastolic volume), afterload (blood pressure) or heart rate to account for the effect of AVP on left ventricular ejection fraction. The seven-day mortality rate was highest in the arginine vasopressin group. Following ischemia/reperfusion injury, we found no change in cardiac V1 Receptor expression but a 40% decrease in Oxytocin Receptor expression. Arginine vasopressin infusion significantly depressed the myocardial function in an ischemia/reperfusion model and increased mortality in comparison with both saline and dobutamine treated animals. The use of vasopressin may be contraindicated in non-vasodilatory shock states associated with significant cardiac injury.
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影响因子：
--
作者：
CHENG, CP;IGARASHI, Y;LITTLE, WC
通讯作者：
LITTLE, WC
影响因子：
38.9
作者：
Holmes, CL;Walley, KR;Russell, JA
通讯作者：
Russell, JA
影响因子：
38.9
作者：
Dünser, MW;Mayr, AJ;Hasibeder, WR
通讯作者：
Hasibeder, WR
影响因子：
7.2
作者：
Hobo, Rutsuko;Netsu, Sachiko;Tsutsumi, Osamu
通讯作者：
Tsutsumi, Osamu
DOI：
10.1186/cc2338
发表时间：
2004-02
期刊：
Critical care (London, England)
影响因子：
--
作者：
Holmes CL;Landry DW;Granton JT
通讯作者：
Granton JT