Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.

Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
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DOI:
10.3201/eid1002.030759
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发表时间:
2004-02
影响因子:
11.8
通讯作者:
Anderson LJ
Anderson LJ
中科院分区:
医学2区
文献类型:
--
作者:
Emery SL;Erdman DD;Bowen MD;Newton BR;Winchell JM;Meyer RF;Tong S;Cook BT;Holloway BP;McCaustland KA;Rota PA;Bankamp B;Lowe LE;Ksiazek TG;Bellini WJ;Anderson LJ

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建立了一种快速检测严重急性呼吸综合征相关冠状病毒(SARS-CoV)的实时逆转录聚合酶链式反应(RT-PCR)方法。该方法基于位于SARS-CoV基因组不同区域的多个引物和探针组,可以将SARS-CoV与其他人和动物冠状病毒区分开来,每次反应的潜在检测下限为10个基因组拷贝。实时荧光定量RT-PCR检测SARS冠状病毒较常规RT-PCR或培养分离更敏感,适用于临床标本中SARS-CoV的检测。该方法的应用将有助于SARS冠状病毒感染的诊断。
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
DOI: 10.1016/s0140-6736(03)13414-9
发表时间: 2003-05-24
期刊: Lancet (London, England)
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