Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
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DOI:
10.3201/eid1002.030759
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发表时间:
2004-02
影响因子:
11.8
通讯作者:
Anderson LJ
中科院分区:
文献类型:
--
作者:
Emery SL;Erdman DD;Bowen MD;Newton BR;Winchell JM;Meyer RF;Tong S;Cook BT;Holloway BP;McCaustland KA;Rota PA;Bankamp B;Lowe LE;Ksiazek TG;Bellini WJ;Anderson LJ
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
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DOI:
10.1016/s0140-6736(03)13414-9
发表时间:
2003-05-24
期刊:
Lancet (London, England)
影响因子:
--
作者:
Ruan YJ;Wei CL;Ee AL;Vega VB;Thoreau H;Su ST;Chia JM;Ng P;Chiu KP;Lim L;Zhang T;Peng CK;Lin EO;Lee NM;Yee SL;Ng LF;Chee RE;Stanton LW;Long PM;Liu ET
通讯作者:
Liu ET
影响因子:
3.1
作者:
Smith, IL;Halpin, K;Smith, GA
通讯作者:
Smith, GA
DOI:
10.1016/s0140-6736(03)13412-5
发表时间:
2003-05-24
期刊:
Lancet (London, England)
影响因子:
--
作者:
Peiris JS;Chu CM;Cheng VC;Chan KS;Hung IF;Poon LL;Law KI;Tang BS;Hon TY;Chan CS;Chan KH;Ng JS;Zheng BJ;Ng WL;Lai RW;Guan Y;Yuen KY;HKU/UCH SARS Study Group
通讯作者:
HKU/UCH SARS Study Group
影响因子:
158.5
作者:
Poutanen, SM;Low, DE;McGeer, AJ
通讯作者:
McGeer, AJ
影响因子:
158.5
作者:
Drosten, C;G端nther, S;Doerr, HW
通讯作者:
Doerr, HW