Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection.
Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection.
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DOI：
10.1016/s0140-6736(03)13414-9
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发表时间：
2003-05-24
期刊：
影响因子：
--
通讯作者：
Liu ET
中科院分区：
文献类型：
--
作者：
Ruan YJ;Wei CL;Ee AL;Vega VB;Thoreau H;Su ST;Chia JM;Ng P;Chiu KP;Lim L;Zhang T;Peng CK;Lin EO;Lee NM;Yee SL;Ng LF;Chee RE;Stanton LW;Long PM;Liu ET
严重急性呼吸综合征(SARS)的病原已被确定为一种新的冠状病毒。对不同分离株的全基因组序列分析可能会为这种新病毒的潜在株系差异提供指示。此外，突变分析将有助于开发有效的疫苗。我们对新加坡出现的指示病例(SIN2500)的培养分离株进行了全基因组测序，这些分离株来自三个主要接触者(SIN2774、SIN2748和SIN2677)和一个二次接触者(SIN2679)。这些序列与来自加拿大(TOR2)、香港(中大-W1和HKU39849)、河内(乌尔巴尼)、广州(GZ01)和北京(BJ01、BJ02、BJ03、BJ04)的分离株进行了比较。我们在14个分离株中鉴定出129个序列变异，其中16个重复变异序列。四个基因座上的常见变异序列定义了SARS病毒的两种不同的基因类型。一个基因型与源自香港M酒店的感染有关，第二个基因包含来自香港、广州和北京的分离株，与M酒店无关(p<；0.0001)。此外，其他常见的序列变异进一步区分了分离株的地理来源，特别是新加坡和北京之间的地理来源。尽管最近爆发了SARS疫情，但根据接触来源历史和地理位置将世界各地的SARS病毒分离株划分为组的遗传特征正在出现。这些特征可以用来追踪感染源。此外，与Spike蛋白S1区域的非保守氨基酸变化相关的一个常见变异表明，免疫压力可能开始影响SARS病毒在人类群体中的进化。在线发布2003年5月9日http://image.thelancet.com/extras/03art4454web.pdf
The cause of severe acute respiratory syndrome (SARS) has been identified as a new coronavirus. Whole genome sequence analysis of various isolates might provide an indication of potential strain differences of this new virus. Moreover, mutation analysis will help to develop effective vaccines. We sequenced the entire SARS viral genome of cultured isolates from the index case (SIN2500) presenting in Singapore, from three primary contacts (SIN2774, SIN2748, and SIN2677), and one secondary contact (SIN2679). These sequences were compared with the isolates from Canada (TOR2), Hong Kong (CUHK-W1 and HKU39849), Hanoi (URBANI), Guangzhou (GZ01), and Beijing (BJ01, BJ02, BJ03, BJ04). We identified 129 sequence variations among the 14 isolates, with 16 recurrent variant sequences. Common variant sequences at four loci define two distinct genotypes of the SARS virus. One genotype was linked with infections originating in Hotel M in Hong Kong, the second contained isolates from Hong Kong, Guangzhou, and Beijing with no association with Hotel M (p<0.0001). Moreover, other common sequence variants further distinguished the geographical origins of the isolates, especially between Singapore and Beijing. Despite the recent onset of the SARS epidemic, genetic signatures are emerging that partition the worldwide SARS viral isolates into groups on the basis of contact source history and geography. These signatures can be used to trace sources of infection. In addition, a common variant associated with a non-conservative aminoacid change in the S1 region of the spike protein, suggests that immunological pressures might be starting to influence the evolution of the SARS virus in human populations. Published online May 9, 2003 http://image.thelancet.com/extras/03art4454web.pdf
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影响因子：
3.7
作者：
Gómez, N;Carrillo, C;Escribano, JM
通讯作者：
Escribano, JM
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1073/pnas.97.13.6974
发表时间：
2000-06-20
影响因子：
11.1
作者：
Bush, RM;Smith, CB;Fitch, WM
通讯作者：
Fitch, WM
影响因子：
5.4
作者：
Rowe, CL;Fleming, JO;Baker, SC
通讯作者：
Baker, SC
影响因子：
5
作者：
Lee, CW;Jackwood, MW
通讯作者：
Jackwood, MW