Galleria mellonella infection model demonstrates high lethality of ST69 and ST127 uropathogenic E. coli.
Galleria mellonella infection model demonstrates high lethality of ST69 and ST127 uropathogenic E. coli.
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DOI：
10.1371/journal.pone.0101547
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Upton M
中科院分区：
文献类型：
--
作者：
Alghoribi MF;Gibreel TM;Dodgson AR;Beatson SA;Upton M
mellonella幼虫是研究细菌致病性的另一种体内模型。在这里，我们用大黄蜂幼虫检测了从5个主要尿路致病性大肠杆菌（UPEC）谱系中分离出来的71株菌株的致病性。用一定剂量的接种量攻毒幼虫，确定50%致死剂量（LD50），并利用Kaplan-Meier图分析存活结果。毒力与携带一组29个毒力因子（VF）相关。接种ST69和ST127分离株（104个菌落形成单位/幼虫）的幼虫死亡率显著高于ST73、ST95和ST131分离株，24小时内杀死50%的幼虫。有趣的是，ST131分离株毒性最小。我们观察到ST127分离株的vf评分显著高于所有其他STs分离株（P≤0.0001），包括ST69 (P<0.02)，但ST127分离株（菌株EC18）是无毒的。与ST127毒株的比较基因组分析显示，菌株EC18的o抗原簇存在IS1介导的缺失，这可能解释了幼虫感染模型中缺乏毒力的原因。幼虫的毒力与血清型或系统发育群无关。本研究表明，大麦香杆菌是研究UPEC菌株毒力的一个很好的工具。这些发现也支持了我们的建议，即应该监测ST127菌株的发病率，因为这些分离株尚未被广泛报道，但它们显然具有比更广泛认识的克隆（包括ST73， ST95或ST131）更大的致病潜力。
Galleria mellonella larvae are an alternative in vivo model for investigating bacterial pathogenicity. Here, we examined the pathogenicity of 71 isolates from five leading uropathogenic E. coli (UPEC) lineages using G. mellonella larvae. Larvae were challenged with a range of inoculum doses to determine the 50% lethal dose (LD50) and for analysis of survival outcome using Kaplan-Meier plots. Virulence was correlated with carriage of a panel of 29 virulence factors (VF). Larvae inoculated with ST69 and ST127 isolates (104 colony-forming units/larvae) showed significantly higher mortality rates than those infected with ST73, ST95 and ST131 isolates, killing 50% of the larvae within 24 hours. Interestingly, ST131 isolates were the least virulent. We observed that ST127 isolates are significantly associated with a higher VF-score than isolates of all other STs tested (P≤0.0001), including ST69 (P<0.02), but one ST127 isolate (strain EC18) was avirulent. Comparative genomic analyses with virulent ST127 strains revealed an IS1 mediated deletion in the O-antigen cluster in strain EC18, which is likely to explain the lack of virulence in the larvae infection model. Virulence in the larvae was not correlated with serotype or phylogenetic group. This study illustrates that G. mellonella are an excellent tool for investigation of the virulence of UPEC strains. The findings also support our suggestion that the incidence of ST127 strains should be monitored, as these isolates have not yet been widely reported, but they clearly have a pathogenic potential greater than that of more widely recognised clones, including ST73, ST95 or ST131.
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影响因子：
9.4
作者：
Bert, Frederic;Johnson, James R.;Nicolas-Chanoine, Marie-Helene
通讯作者：
Nicolas-Chanoine, Marie-Helene
影响因子：
5.2
作者：
Croxall, Gemma;Hale, James;McNally, Alan
通讯作者：
McNally, Alan
影响因子：
3.1
作者：
Dobrindt, U;Blum-Oehler, G;Hacker, J
通讯作者：
Hacker, J
影响因子：
3.8
作者：
Brown, Susan E.;Howard, Antoinette;East, Peter D.
通讯作者：
East, Peter D.
影响因子：
2.8
作者：
Champion, Olivia L.;Cooper, Ian A. M.;Titball, Richard W.
通讯作者：
Titball, Richard W.