Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
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DOI：
10.1371/journal.pone.0033241
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Romano A
中科院分区：
文献类型：
--
作者：
Schreinemacher MH;Backes WH;Slenter JM;Xanthoulea S;Delvoux B;van Winden L;Beets-Tan RG;Evers JL;Dunselman GA;Romano A
Endometriosis is defined as the presence of endometrial tissue outside the uterus. It affects 10–15% of women during reproductive age and has a big personal and social impact due to chronic pelvic pain, subfertility, loss of work-hours and medical costs. Such conditions are exacerbated by the fact that the correct diagnosis is made as late as 8–11 years after symptom presentation. This is due to the lack of a reliable non-invasive diagnostic test and the fact that the reference diagnostic standard is laparoscopy (invasive, expensive and not without risks). High-molecular weight gadofosveset-trisodium is used as contrast agent in Magnetic Resonance Imaging (MRI). Since it extravasates from hyperpermeable vessels more easily than from mature blood vessels, this contrast agent detects angiogenesis efficiently. Endometriosis has high angiogenic activity. Therefore, we have tested the possibility to detect endometriosis non-invasively using Dynamic Contrast-Enhanced MRI (DCE-MRI) and gadofosveset-trisodium as a contrast agent in a mouse model. Endometriotic lesions were surgically induced in nine mice by autologous transplantation. Three weeks after lesion induction, mice were scanned by DCE-MRI. Dynamic image analysis showed that the rates of uptake (inwash), persistence and outwash of the contrast agent were different between endometriosis and control tissues (large blood vessels and back muscle). Due to the extensive angiogenesis in induced lesions, the contrast agent persisted longer in endometriotic than control tissues, thus enhancing the MRI signal intensity. DCE-MRI was repeated five weeks after lesion induction, and contrast enhancement was similar to that observed three weeks after endometriosis induction. The endothelial-cell marker CD31 and the pericyte marker α-smooth-muscle-actin (mature vessels) were detected with immunohistochemistry and confirmed that endometriotic lesions had significantly higher prevalence of new vessels (CD31 only positive) than the uterus and control tissues. The diagnostic value of gadofosveset-trisodium to detect endometriosis should be tested in human settings.
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DOI：
10.1177/1933719108330568
发表时间：
2009-04
期刊：
Reproductive sciences (Thousand Oaks, Calif.)
影响因子：
--
作者：
Rogers PA;D'Hooghe TM;Fazleabas A;Gargett CE;Giudice LC;Montgomery GW;Rombauts L;Salamonsen LA;Zondervan KT
通讯作者：
Zondervan KT
DOI：
10.1016/j.bpobgyn.2004.03.002
发表时间：
2004-04-01
影响因子：
5.5
作者：
Brosens, I;Puttemans, P;Gordts, S
通讯作者：
Gordts, S
影响因子：
5.9
作者：
Essig, Marco;Nikolaou, Konstantin;Meaney, James F. M.
通讯作者：
Meaney, James F. M.
影响因子：
--
作者：
Marcal, Leonardo;Nothaft, Maria Angela;Choi, Haesun
通讯作者：
Choi, Haesun
影响因子：
2.9
作者：
Goyen M
通讯作者：
Goyen M