Transferrin, derived from an OKT8-positive subpopulation of T lymphocytes, suppresses the production of granulocyte-macrophage colony- stimulatory factors from mitogen-activated T lymphocytes
Transferrin, derived from an OKT8-positive subpopulation of T lymphocytes, suppresses the production of granulocyte-macrophage colony- stimulatory factors from mitogen-activated T lymphocytes
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转铁蛋白源自 OKT8 阳性 T 淋巴细胞亚群，可抑制有丝分裂原激活的 T 淋巴细胞产生粒细胞-巨噬细胞集落刺激因子
DOI：
10.1182/blood.v62.1.37.bloodjournal62137
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发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
J. Bognacki
中科院分区：
文献类型：
--
作者：
H. Broxmeyer;Lien Lu;J. Bognacki
纯化的人转铁蛋白在铁或锌饱和后，会减少受植物血凝素或刀豆球蛋白 A 刺激的人 T 淋巴细胞产生粒细胞巨噬细胞集落刺激因子 (GM-CSF)。铁饱和的转铁蛋白比锌饱和的转铁蛋白活性更高。对于铜饱和转铁蛋白或脱铁转铁蛋白没有观察到这种效果，并且无论在存在或不存在血清的情况下产生GM-CSF，都观察到抑制效果。如果用抗转铁蛋白受体的单克隆抗体预处理淋巴细胞，则未观察到转铁蛋白的抑制作用。转铁蛋白对粒细胞-巨噬细胞集落或簇形成细胞 (CFU-GM) 或预先形成的 GM-CSF 没有直接影响。转铁蛋白抑制活性仅由具有 OKT8+ 抗原表型的 T 淋巴细胞亚群产生和释放。 6-17 小时后，首次检测到 OKT8+ 淋巴细胞将这种活性释放到 37°C 的培养基中，但产生 GM-CSF 的淋巴细胞对转铁蛋白抑制活性的反应能力仅在将淋巴细胞置于 37°C 的前 3 小时内才明显。这些研究表明，反馈相互作用仅限于 T 淋巴细胞谱系的细胞，可能与骨髓生成的调节有关。
Purified human transferrin, when saturated with iron or zinc, decreased the production of granulocyte-macrophage colony-stimulating factors (GM- CSF) by human T lymphocytes that had been stimulated by phytohemagglutin or concanavalin-A. The iron-saturated transferrin was more active than the zinc-saturated transferrin. This effect was not seen for copper-saturated transferrin or for apotransferrin, and the inhibitory effect was seen whether production of GM-CSF occurred in the absence or presence of serum. If the lymphocytes were pretreated with monoclonal antibody against transferrin receptors, no suppressive effect with transferrin was seen. Transferrin did not have a direct effect on the granulocyte-macrophage colony or cluster-forming cells (CFU-GM) or on preformed GM-CSF. Transferrin-inhibitory activity was produced and released only from a subpopulation of T lymphocytes that had the OKT8+ antigenic phenotype. Release of this activity from OKT8+ lymphocytes, into culture medium at 37 degrees C, was first detected after 6–17 hr, but the capacity of the GM-CSF-producing lymphocytes to respond to transferrin-inhibitory activity was apparent only within the first 3 hr of placing the lymphocytes at 37 degrees C. These studies demonstrate feedback interactions confined to cells of the T-lymphocyte lineage that may be of relevance to the regulation of myelopoiesis.
DOI：
10.1172/jci110649
发表时间：
1982-09
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
L. Pelus
通讯作者：
L. Pelus
一种单克隆抗体，可检测人红系前体细胞中转铁蛋白受体的表达。
影响因子：
20.3
作者：
Lebman,D;Trucco,M;Bottero,L;Lange,B;Pessano,S;Rovera,G
通讯作者：
Rovera,G
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Beller,DI;Unanue,ER
通讯作者：
Unanue,ER