Functional expression and germline atransmission of a human chromosome fragment in chimaeric mice
Functional expression and germline atransmission of a human chromosome fragment in chimaeric mice
复制标题
嵌合小鼠中人类染色体片段的功能表达和种系传递
作者：
K. Tomizuka;H. Yoshida;H. Uejima;H. Kugoh;Kaoru Sato;A. Ohguma;M. Hayasaka;K. Hanaoka;M. Oshimura;I. Ishida
采用微细胞介导的染色体转移（MMCT）技术，将人染色体或正常成纤维细胞染色体片段导入小鼠胚胎干细胞（ES细胞）中，获得嵌合体小鼠。转移的染色体稳定保留，人基因，包括免疫球蛋白（IG）κ，重链，λ基因，在成人嵌合体组织中以适当的组织特异性方式表达。在人类染色体（hChr.）2-通过对衍生片段的分析，发现它通过生殖系传播给后代。我们的研究表明，MMCT允许引入非常大量的外源遗传物质到小鼠。这一新的方法将有助于在体内对人类基因组进行功能分析。
Human chromosomes or chromosome fragments derived from normal fibroblasts were introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Transferred chromosomes were stably retained, and human genes, including immunoglobulin (Ig) κ, heavy, λ genes, were expressed in proper tissue-specific manner in adult chimaeric tissues. In the case of a human chromosome (hChr.) 2-derived fragment, it was found to be transmitted to the offspring through the germline. Our study demonstrates that MMCT allows for introduction of very large amounts of foreign genetic material into mice. This novel procedure will facilitate the functional analyses of human genomes in vivo.
影响因子：
3.5
作者：
HUNT, P;LEMAIRE, R;MROZ, K
通讯作者：
MROZ, K
影响因子：
3.9
作者：
Schoneveld, OJLM;Gaemers, IC;Lamers, WH
通讯作者：
Lamers, WH
鉴定人类肌红蛋白基因 5 侧翼区域内的肌肉特异性增强子。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Devlin,BH;Wefald,FC;Kraus,WE;Bernard,TS;Williams,RS
通讯作者：
Williams,RS