Selective Germline Genome Edited Pig Meninges Grafts for the Abdominal Wall Closure in Damage Control Surgery
Selective Germline Genome Edited Pig Meninges Grafts for the Abdominal Wall Closure in Damage Control Surgery
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选择性种系基因组编辑的猪脑膜移植物用于损伤控制手术中的腹壁闭合
DOI：
10.1101/2020.02.12.946178
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Gang Wang
中科院分区：
文献类型：
--
作者：
Lijin Zou;Youlai Zhang;Ying He;Hui Yu;Jun Chen;Delong Liu;Sixiong Lin;Manman Gao;Gang Zhong;Weicheng Lei;Guangqian Zhou;Xuenong Zou;Kai Li;Yin Yu;Gaofeng Zha;Linxian Li;Yuanlin Zeng;Jianfei Wang;Gang Wang
腹壁缺损的重建仍然是外科手术中的一大挑战，特别是在紧急和危及生命的情况下，筋膜肌肉不足或缺损张力过大。事实上，损伤控制手术的概念已经在创伤和非创伤手术环境的管理中得到了发展。该策略需要简化手术并迅速返回重症监护室（ICU）进行积极的复苏。在损伤控制剖腹手术中，患者留有开放的腹部或暂时关闭腹部，并计划返回手术室进行最终手术。到目前为止，人们已利用各种技术来实现早期临时腹部闭合，但对于腹壁重建的理想方法或材料尚未达成明确共识。我们最近成功地创建了选择性种系基因组编辑猪（SGGEP），在这里，我们的目的是探索在兔模型中用SGGEP脑膜移植物（SGGEP-MG）体内重建腹壁的可行性。我们的结果表明，SGGEP-MG可以很好地恢复缺损的完整性。植入7周后，未观察到疝形成迹象，移植物实现了血管再通，缺损得到了良好修复。在组织学上，移植物和宿主之间的边界非常好地整合，并且没有强烈的炎症反应。因此，这种关闭可以帮助恢复水和电解质平衡，并在损伤控制手术中抑制全身炎症反应，而ADM移植物不能建立与SGGEP-MG相同的再血管化。可以得出结论，SGGEP的脑膜可以作为一种高质量的替代方案，用于恢复腹壁的完整性，特别是用于损伤控制手术。
Reconstruction of abdominal wall defects is still a big challenge in surgery, especially where there is insufficient fascia muscular or excessive tension of the defects in emergency and life-threatening scenarios. Indeed, the concept of damage control surgery has been advanced in the management of both traumatic and nontraumatic surgical settings. The strategy requires abridged surgery and quick back to intensive care units (ICU) for aggressive resuscitation. In the damage control laparotomy, patients are left with open abdomen or provisional closure of the abdomen with a planned return to the operating room for definitive surgery. So far, various techniques have been utilized to achieve early temporary abdominal closure, but there is no clear consensus on the ideal method or material for abdominal wall reconstruction. We recently successfully created the selective germline genome-edited pig (SGGEP) and here we aimed to explore the feasibility of in vivo reconstruction of the abdominal wall in a rabbit model with SGGEP meninges grafts (SGGEP-MGs). Our result showed that the SGGEP-MGs could restore the integrity of the defect very well. After 7 weeks of engraftment, there was no sign of herniation observed, the grafts were re-vascularized, and the defects were well repaired. Histologically, the boundary between the graft and the host was very well integrated and there was no strong inflammatory response. Therefore, this kind of closure could help restore the fluid and electrolyte balance and to dampen systemic inflammatory response in damge control surgery while ADM graft failed to establish re-vascularization as the same as the SGGEP-MG. It is concluded that the meninges of SGGEP could serve as a high-quality alternative for restoring the integrity of the abdominal wall, especially for damage control surgery.
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影响因子：
3
作者：
E. Jacobs;F. W. Blaisdell;A. D. Hall
通讯作者：
E. Jacobs;F. W. Blaisdell;A. D. Hall
DOI：
10.1111/j.1469-0691.2005.01081.x
发表时间：
2005-04
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Cantón R
通讯作者：
Cantón R
影响因子：
3
作者：
J. Pridgen;C. Tennison
通讯作者：
J. Pridgen;C. Tennison
影响因子：
0.9
作者：
Shazia Abid;K. El-Hayek
通讯作者：
Shazia Abid;K. El-Hayek
DOI：
10.1007/s00464-006-9157-9
发表时间：
2007-04-01
影响因子：
3.1
作者：
Jacob, B. P.;Hogle, N. J.;Fowler, D. L.
通讯作者：
Fowler, D. L.