Does your gene need a background check? How genetic background impacts the analysis of mutations, genes, and evolution.
Does your gene need a background check? How genetic background impacts the analysis of mutations, genes, and evolution.
复制标题
DOI：
10.1016/j.tig.2013.01.009
复制
发表时间：
2013-06
影响因子：
11.4
通讯作者：
Dworkin, Ian
中科院分区：
文献类型：
--
作者：
Chandler, Christopher H.;Chari, Sudarshan;Dworkin, Ian
关键词：
遗传分析的前提是表型变异和等位基因变异之间存在因果联系。然而，长期以来一直有文献证明，突变表型不是单一DNA损伤的简单结果，而是局部等位基因与其他基因和环境相互作用的结果。尽管专注于个体突变和同基因对照菌株的严格实验方法促进了遗传学和相关领域的惊人进步，但回顾一下就会发现，我们目前对等位基因效应的理解忽略了一个巨大的复杂性。有了传统的遗传分析和它们提供的基础知识，我们认为，回到基因功能未被充分探索的方面并拥抱遗传效应的上下文相关性质的时机和工具已经成熟。我们断言，要广泛理解遗传效应和等位基因的进化动态，就需要确定突变结果如何取决于“野生型”遗传背景。此外，我们还讨论了如何最好地利用遗传背景效应来拓宽遗传研究项目。
The premise of genetic analysis is that a causal link exists between phenotypic and allelic variation. Yet it has long been documented that mutant phenotypes are not a simple result of a single DNA lesion, but rather are due to interactions of the focal allele with other genes and the environment. Although an experimentally rigorous approach focused on individual mutations and isogenic control strains has facilitated amazing progress within genetics and related fields, a glimpse back suggests that a vast complexity has been omitted from our current understanding of allelic effects. Armed with traditional genetic analyses and the foundational knowledge they have provided, we argue that the time and tools are ripe to return to the under-explored aspects of gene function and embrace the context-dependent nature of genetic effects. We assert that a broad understanding of genetic effects and the evolutionary dynamics of alleles requires identifying how mutational outcomes depend upon the “wild-type” genetic background. Furthermore, we discuss how best to exploit genetic background effects to broaden genetic research programs.
登录
查看更多内容
影响因子：
64.8
作者：
Burnett, Camilla;Valentini, Sara;Cabreiro, Filipe;Goss, Martin;Somogyvari, Milan;Piper, Matthew D.;Hoddinott, Matthew;Sutphin, George L.;Leko, Vid;McElwee, Joshua J.;Vazquez-Manrique, Rafael P.;Orfila, Anne-Marie;Ackerman, Daniel;Au, Catherine;Vinti, Giovanna;Riesen, Michele;Howard, Ken;Neri, Christian;Bedalov, Antonio;Kaeberlein, Matt;Soti, Csaba;Partridge, Linda;Gems, David
通讯作者：
Gems, David
DOI：
10.1126/science.1187816
发表时间：
2010-06-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bloom JD;Gong LI;Baltimore D
通讯作者：
Baltimore D
影响因子：
3.3
作者：
Arbuthnott, Devin;Rundle, Howard D.
通讯作者：
Rundle, Howard D.
影响因子：
9.8
作者：
Duveau F;Félix MA
通讯作者：
Félix MA
影响因子：
3.7
作者：
Alic N;Hoddinott MP;Foley A;Slack C;Piper MD;Partridge L
通讯作者：
Partridge L