Subcellular distribution, synthesis, and release of carcinoembryonic antigen in cultured human colon adenocarcinoma cell lines.
Subcellular distribution, synthesis, and release of carcinoembryonic antigen in cultured human colon adenocarcinoma cell lines.
复制标题
培养的人结肠腺癌细胞系中癌胚抗原的亚细胞分布、合成和释放。
作者：
Z. Shi;D. Tsao;Y. Kim
本文研究了9种人结肠癌细胞系癌胚抗原（CEA）的含量及其亚细胞分布。不同结肠癌细胞系间CEA含量差异较大。分化良好的结肠癌细胞系（LS 174 T和SKCO-1）含有最高的CEA活性，其比分化较低的细胞（SW 620、SW 480和HRT 18）高35至60倍。在所有9个细胞系中，超过80%的CEA与细胞膜结合。随着细胞密度的增加，SW 1116、HCT 8和HCT 48细胞中每个细胞的CEA含量增加，而SW 620、HRT 18和HT 29细胞中没有观察到变化。在SW 480、LS 174 T和SKCO-1细胞中，CEA含量实际上随着细胞密度的增加而降低。研究CEA在细胞中的合成及其在8天内释放到培养基中的情况表明，LS 174 T和SKCO-1细胞在最大细胞密度下的CEA合成速率分别降低到低细胞密度下的15%和50%。相反，这两种细胞系的CEA释放到培养基中的速率在最大值时高于低细胞密度时。对于HCT 48细胞，随着细胞密度的增加，CEA合成速率的增加显著地提高了细胞CEA水平。通过研究N-乙酰基[3 H]-葡糖胺掺入LS 174 T和HCT 48细胞系中的细胞CEA的速率证实了这些观察结果。对于LS 174 T，在生长的指数期，放射性掺入CEA的速率大于稳定期，而对于HCT 48细胞，观察到相反的情况。本研究表明，在不同的人结肠癌细胞系之间CEA含量有很大的差异，并且它主要与膜部分相关。CEA的合成和释放速率在不同的细胞系中也不同。生长阶段对这些人结肠癌细胞系中CEA含量、合成速率和CEA释放具有不同的影响。
The content of carcinoembryonic antigen (CEA) and its subcellular distribution were studied in nine human colon carcinoma cell lines. A great variation in CEA content was found among different colon cancer cell lines. Well-differentiated colon cancer cell lines (LS174T and SKCO-1) contained the highest CEA activity which was 35 to 60 times greater than that of less well-differentiated cells (SW620, SW480, and HRT18). More than 80% of the CEA was associated with the cell membrane in all nine cell lines. With increasing cell density, the CEA content per cell was found to increase in SW1116, HCT8, and HCT48 cells, while no change was observed in SW620, HRT18, and HT29 cells. In SW480, LS174T, and SKCO-1 cells, CEA content actually decreased with increasing cell density. Investigation of the synthesis of CEA in cells and its release into the medium over an 8-day period showed that the rate of CEA synthesis at maximum cell density for LS174T and SKCO-1 cells decreased to 15 and 50% of that at low cell density, respectively. In contrast, the rate of CEA release into medium by these two cell lines was higher at maximum than at low cell density. For HCT48 cells, the increased rate of CEA synthesis with increasing cell density markedly elevated cellular CEA levels. These observations were confirmed by studying the rate of incorporation of N-acetyl[3H]-glucosamine into cellular CEA in LS174T and HCT48 cell lines. The rate of incorporation of radioactivity into CEA was greater during the exponential phase of growth than during the stationary phase for LS174T, while the opposite was observed with HCT48 cells. This study indicated that there is a great variation in CEA content among different human colon cancer cell lines and that it is associated predominantly with the membrane fraction. The rate of synthesis and release of CEA also varied among different cell lines. The growth phase had a varied effect on the CEA content, the rate of synthesis, and the release of CEA in these human colon cancer cell lines.
良性和恶性结直肠组织中癌胚抗原的免疫细胞化学定位。
影响因子：
3.5
作者：
O'Brien,MJ;Zamcheck,N;Burke,B;Kirkham,SE;Saravis,CA;Gottlieb,LS
通讯作者：
Gottlieb,LS