Reuse of malaria rapid diagnostic tests for amplicon deep sequencing to estimate Plasmodium falciparum transmission intensity in western Uganda.
Reuse of malaria rapid diagnostic tests for amplicon deep sequencing to estimate Plasmodium falciparum transmission intensity in western Uganda.
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DOI：
10.1038/s41598-018-28534-3
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发表时间：
2018-07-05
影响因子：
4.6
通讯作者：
Juliano JJ
中科院分区：
文献类型：
--
作者：
Boyce RM;Hathaway N;Fulton T;Reyes R;Matte M;Ntaro M;Mulogo E;Waltmann A;Bailey JA;Siedner MJ;Juliano JJ
分子技术并未常规用于疟疾监测，而以社区为基础的横断面寄生虫调查需要大量资源。在这里，我们描述了在单一设施收集的疟疾快速诊断测试（RDTs）作为测序源材料的一种新用途，以估计在一个相对较大的集水区的疟疾传播强度。我们从rdt中提取恶性疟原虫DNA，然后利用靶向扩增子深度测序技术扩增并测序了一个区域的根尖膜抗原1 （pfama1）。我们确定了每个样本的感染多样性（MOI），并检查了与人口统计学、临床和空间因素的关联。287份样本中有223份（77.7%）成功分型。我们证明了MOI与海拔高度呈反比关系，来自海拔最高村庄的个体感染的复杂性约为海拔最低村庄感染的一半（MOI 1.85 vs. 3.51, AOR 0.25, 95% CI 0.09-0.65, p = 0.004）。这项研究证明了使用常规收集的rdt进行疟疾分子监测的可行性和有效性，并且具有现实实用性，特别是随着高通量测序成本的持续下降。
Molecular techniques are not routinely employed for malaria surveillance, while cross-sectional, community-based parasite surveys require significant resources. Here, we describe a novel use of malaria rapid diagnostic tests (RDTs) collected at a single facility as source material for sequencing to esimtate malaria transmission intensity across a relatively large catchment area. We extracted Plasmodium falciparum DNA from RDTs, then amplified and sequenced a region of the apical membrane antigen 1 (pfama1) using targeted amplicon deep sequencing. We determined the multiplicity of infection (MOI) for each sample and examined associations with demographic, clinical, and spatial factors. We successfully genotyped 223 of 287 (77.7%) of the samples. We demonstrated an inverse relationship between the MOI and elevation with individuals presenting from the highest elevation villages harboring infections approximately half as complex as those from the lowest (MOI 1.85 vs. 3.51, AOR 0.25, 95% CI 0.09–0.65, p = 0.004). This study demonstrates the feasibility and validity of using routinely-collected RDTs for molecular surveillance of malaria and has real-world utility, especially as the cost of high-throughpout sequencing continues to decline.
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影响因子：
3
作者：
Cnops L;Boderie M;Gillet P;Van Esbroeck M;Jacobs J
通讯作者：
Jacobs J
影响因子：
56.3
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Snow, Robert W.
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影响因子：
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作者：
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通讯作者：
Meshnick, Steven R.
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作者：
Engelbrecht, F;Tögel, E;Felger, I
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