Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion.
Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion.
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DOI：
10.1186/1471-2407-8-287
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发表时间：
2008-10-06
期刊：
影响因子：
3.8
通讯作者：
Iwase H
中科院分区：
文献类型：
--
作者：
Okumura Y;Yamamoto Y;Zhang Z;Toyama T;Kawasoe T;Ibusuki M;Honda Y;Iyama K;Yamashita H;Iwase H
乳腺X线摄影在乳腺癌筛查中的广泛应用导致越来越多的导管原位癌（DCIS）患者被发现。直径小于或等于1 mm的病灶浸润区的乳腺DCIS定义为DCIS伴微浸润，DCIS-Mi。识别DCIS和DCIS-Mi之间的生物学差异可能有助于理解DCIS进展为侵袭性的性质和原因。在这项研究中，使用切除的乳腺癌组织，我们比较了纯DCIS（52例）和DCIS-Mi（28例）导管内病变的病理结果，生物学因素，凋亡相关蛋白的表达和增殖能力，通过使用免疫组化和TdT介导的dUTP-生物素缺口末端标记（TUNEL）法。就雌激素受体、孕激素受体和人表皮生长因子受体2型的水平而言，DCIS和DCIS-Mi之间的生物学因素没有差异。与DCIS相比，DCIS-Mi中肿瘤坏死率及Survivin、Bax阳性表达率明显增高。此外，凋亡指数，Ki-67指数，和阳性Bcl-2免疫标记的DCIS-Mi往往高于DCIS。多因素分析显示，肿瘤坏死和Survivin阳性表达是影响肿瘤侵袭的独立因素。与DCIS相比，DCIS-Mi的特征在于导管内病变内的细胞增殖能力略微升高和凋亡增强，这两者被认为促进细胞坏死灶的形成。此外，生存素的差异表达可能有助于决定对治疗的反应，并可能具有一定的预后意义。
Widespread use of mammography in breast cancer screening has led to the identification of increasing numbers of patients with ductal carcinoma in situ (DCIS). DCIS of the breast with an area of focal invasion 1 mm or less in diameter is defined as DCIS with microinvasion, DCIS-Mi. Identification of biological differences between DCIS and DCIS-Mi may aid in understanding of the nature and causes of the progression of DCIS to invasiveness. In this study, using resected breast cancer tissues, we compared pure DCIS (52 cases) and DCIS-Mi (28 cases) with regard to pathological findings of intraductal lesions, biological factors, apoptosis-related protein expression, and proliferative capacity through the use of immunohistochemistry and the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. There were no differences in biological factors between DCIS and DCIS-Mi, with respect to levels of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor type 2. The frequency of necrosis and positive expression ratio of survivin and Bax were significantly higher in DCIS-Mi than in DCIS. In addition, apoptotic index, Ki-67 index, and positive Bcl-2 immunolabeling tended to be higher in DCIS-Mi than in DCIS. Multivariate analysis revealed that the presence of necrosis and positive survivin expression were independent factors associated with invasion. Compared with DCIS, DCIS-Mi is characterized by a slightly elevated cell proliferation capacity and enhanced apoptosis within the intraductal lesion, both of which are thought to promote the formation of cell necrotic foci. Furthermore, the differential expression of survivin may serve in deciding the response to therapy and may have some prognostic significance.
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Survivin 是预后不良的乳腺癌患者短期生存的独立预测因子。
影响因子：
8.8
作者：
通讯作者：
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影响因子：
8.8
作者：
Bijker N;Peterse JL;Duchateau L;Robanus-Maandag EC;Bosch CA;Duval C;Pilotti S;van de Vijver MJ
通讯作者：
van de Vijver MJ
DOI：
10.1007/bf01008011
发表时间：
1997-01-01
期刊：
VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
影响因子：
--
作者：
Kapucuoglu, N;Losi, L;Eusebi, V
通讯作者：
Eusebi, V
影响因子：
3.8
作者：
Mommers, ECM;van Diest, PJ;Baak, JPA
通讯作者：
Baak, JPA
影响因子：
3.5
作者：
Iwase, H;Omoto, Y;Kobayashi, S
通讯作者：
Kobayashi, S