The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14
The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14
复制标题
诱导型细胞毒性T淋巴细胞相关基因转录本CTLA-1序列和基因定位于小鼠14号染色体
作者：
J. Brunet;M. Dosseto;F. Denizot;M. Mattei;W. Clark;T. Haqqi;P. Ferrier;M. Nabholz;A. Schmitt;M. Luciani;P. Golstein
研究 T 细胞介导的细胞毒性1-3 机制的经典现象学方法现在已经让位于寻找参与该功能的分子。这是通过对来自细胞毒性细胞的材料进行亚细胞和生化分级分离4,5，或通过细胞毒性抑制单克隆抗体识别的分子的表征来尝试的（参见参考文献6的综述）。还可以通过检测相应的信使RNA转录物来鉴定在细胞毒性中起作用的分子。这种方法可能包括，作为第一步，寻找对细胞毒性 T 细胞尽可能特异的转录本；其次才能研究它们与细胞毒性的实际相关性。我们在这里报告了差异互补DNA库的制备和系统筛选，其中我们检测到三种不同的信使RNA转录本（CTLA-1、CTLA-2和CTLA-3）存在于各种细胞毒性T细胞中，但在一系列非细胞毒性淋巴细胞中不存在（或较少存在）。我们描述了这些转录物的共诱导性以及胸腺细胞和杂交瘤细胞中的细胞毒性、CTLA-1 cDNA 的序列、其与丝氨酸酯酶的蛋白质同源性以及相应基因在小鼠 14 号染色体上的定位。
Classical phenomenological approaches to the study of the mechanism of T-cell-mediated cytotoxicity1–3 have now given way to a search for molecules involved in this function; this is attempted either by subcellular and biochemical fractionation of material from cytotoxic cells4,5, or through the characterization of molecules recognized by cytotoxicity-inhibiting monoclonal antibodies (see ref. 6 for a review). Molecules having a role in cytotoxi-city may also be identified by detecting the corresponding messenger RNA transcripts. Such an approach may include, as a first step, the search for transcripts as specific as possible to cytotoxic T cells; only secondarily can their actual relevance to cytotoxicity be investigated. We report here the preparation and systematic screening of a differential complementary DNA bank, in which we detected three distinct messenger RNA transcripts (CTLA-1, CTLA-2 and CTLA-3) present in various cytotoxic T cells but not (or less so) in a range of non-cytotoxic lymphoid cells. We describe the co-inducibility of these transcripts and of cytoxicity in thymocytes and hybridoma cells, the sequence of CTLA-1 cDNA, its protein homology with serine esterases and the localization of the corresponding gene to mouse chromosome 14.
小鼠 T 细胞受体 α 链基因和 T 细胞 γ 基因的染色体位置。
DOI：
10.1126/science.3918347
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kranz,DM;Saito,H;Disteche,CM;Swisshelm,K;Pravtcheva,D;Ruddle,FH;Eisen,HN;Tonegawa,S
通讯作者：
Tonegawa,S