Changes in transcriptomic and metabolomic profiles of morphotypes of Ophiocordyceps sinensis within the hemocoel of its host larvae, Thitarodes xiaojinensis.
Changes in transcriptomic and metabolomic profiles of morphotypes of Ophiocordyceps sinensis within the hemocoel of its host larvae, Thitarodes xiaojinensis.
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冬虫夏草在其宿主幼虫小金丝毛虫血腔内的形态型转录组学和代谢组学特征的变化
DOI：
10.1186/s12864-020-07209-2
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发表时间：
2020-11-11
期刊：
影响因子：
4.4
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Li M;Meng Q;Zhang H;Shu R;Zhao Y;Wu P;Li X;Zhou G;Qin Q;Zhang J
背景冬虫夏草Ophiocordyceps sinensis(Berk.)是一种著名的昆虫病原药用真菌。它寄生和木乃伊地下幽灵蛾幼虫，产生一个子实体，命名为冬虫夏草。对于这种真菌，中华稻瘟病菌经历了一个长达5个月的生物营养营养生长期。在营养生长过程中，在寄主血囊中先后出现了3/4种形态，即酵母状囊孢子(分为增殖期(BP)和稳定期(BS))、前菌丝(Prehy)和菌丝(Hy)。这种特殊的形态发生通过形态和超微结构的观察已经被阐明，但其分子基础仍然是神秘的。本研究通过BP、BS、Prehy和Hy四个阶段的转录组和代谢组谱分析，研究了调控植物营养发育的关键基因、代谢产物和信号转导途径。结果研究了在不同发育阶段调节不同细胞内过程的分子事件和代谢途径。对四个阶段差异表达基因的聚类分析揭示了阶段特定丰富的途径。代谢组分析表明，在受试发育阶段Ofo的碳代谢和几种氨基酸的生物合成受到显著干扰。在宿主血腔中有罪恶。与酿酒酵母MAPK级联序列同源的基因在胚芽孢子向菌丝的转变过程中显著上调。调节蛋白Sho1的上调表明营养饥饿在MAPK途径的激活和丝状细胞的生长中起作用。此外，几个脂肪酸合成基因的上调及其相应产物在BS样本中的积累可能解释了BS比BP观察到更多的脂滴。再加上Prehy和Hy阶段脂肪酸降解的上调，推测脂质的积累和动员在丝状细胞的发育中起着重要的作用。结论这是第一个全面描述Fo的发育转录和代谢组学的报告。我们的发现为了解寄主血腔中真菌形态变化的关键途径和中枢基因提供了新的视角，并有望指导未来昆虫病原真菌体内形态发生和形态类型变化的研究。
BackgroundOphiocordyceps sinensis(Berk.) is a well-known entomopathogenic and medicinal fungus. It parasitizes and mummifies the underground ghost moth larvae to produce a fruiting body named Chinese cordyceps. Specific for the fungus,O. sinensisexperiences a biotrophic vegetative growth period spanning over 5 months. During this vegetative growth, it appears successively in the host hemocoel in three/four morphotypes, namely, the yeast-like blastospores (subdivided into proliferative (BP) and stationary phase (BS)), prehyphae (PreHy) and the hyphae (Hy). This peculiar morphogenesis has been elucidated through morphological and ultrastructural observations, but its molecular basis remains cryptic. In this study, transcriptome and metabolome profiling of BP, BS, PreHy and Hy stages were performed to characterize the key genes, metabolites, and signaling pathways that regulated the vegetative development ofO. sinensisinThitarodes xiaojinensislarva.ResultsThe molecular events and metabolic pathways that regulated different intracellular processes at various stages were examined. Cluster analyses of differentially expressed genes across the four stages revealed the stage specifically enriched pathways. Analysis of metabolome profiles showed that carbon metabolism and several amino acids biosynthesis were significantly perturbed during the tested development stages ofO. sinensisin the host hemocoel. Genes homologous toSaccharomyces cerevisiaeMAPK cascade were significantly up-regulated during the transition from blastospore to hypha. The up-regulation of Sho1, a regulator protein, suggested nutrient starvation act a role in activation of MAPK pathway and filamentous growth. In addition, up-regulation of several fatty acid synthesis genes and their corresponding products accumulation in the samples of BS might explain more lipid droplets were observed in BS than in BP. Coupled with the up-regulation of fatty acid degradation during PreHy and Hy stages, it is presumed that lipid accumulation and mobilization play important roles in filamentous development.ConclusionsThis is the first report comprehensively describing developmental transcriptomics and metabolomics ofO. sinensisin vivo.Our findings provide new perspectives into the key pathways and hub genes involved in morphological changes of fungus developed in the hemocoel of its host, and are expected to guide future studies on morphogenesis and morphotype changes of entomopathogenic fungi in vivo.
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影响因子：
2.5
作者：
ERREDE, B;CADE, RM;MATSUMOTO, K
通讯作者：
MATSUMOTO, K
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.3
作者：
Cutler, NS;Pan, XW;Cardenas, ME
通讯作者：
Cardenas, ME
影响因子：
48
作者：
Kim D;Langmead B;Salzberg SL
通讯作者：
Salzberg SL
DOI：
10.1073/pnas.240345197
发表时间：
2000-12-05
影响因子：
11.1
作者：
Cullen, PJ;Sprague, GF
通讯作者：
Sprague, GF