Combining pharmacology and whole-cell patch recording from CNS neurons, in vivo.
Combining pharmacology and whole-cell patch recording from CNS neurons, in vivo.
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DOI：
10.1016/j.jneumeth.2012.12.003
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发表时间：
2013-02-15
影响因子：
3
通讯作者：
Leary, Christopher J.
中科院分区：
文献类型：
--
作者：
Rose, Gary J.;Alluri, Rishi K.;Vasquez-Opazo, Gustavo A.;Odom, Stephen E.;Graham, Jalina A.;Leary, Christopher J.
关键词：
Whole-cell patch neurophysiology and pharmacological manipulations have provided unprecedented insight into the functions of central neurons, but their combined use has been largely restricted to in vitro preparations. We describe a method for performing whole-cell patch recording and focal application of pharmacological agents in vivo. A key feature of this technique involves iontophoresis of glutamate to establish proximity of drug and recording pipettes. We show data from iontophoresis of glutamate during extracellular and whole-cell recordings made in vivo from auditory neurons in the midbrain of the leopard frog, Rana pipiens, and the effects of blocking GABAA receptors while making a whole-cell recording. This methodology should accelerate our understanding of the roles of particular neurotransmitter systems in normal and pathological conditions, and facilitate investigation of the in vivo effects of drugs and the mechanisms underlying computations.
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DOI：
10.1523/jneurosci.0460-12.2012
发表时间：
2012-08-08
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Duguid I;Branco T;London M;Chadderton P;Häusser M
通讯作者：
Häusser M
影响因子：
5.3
作者：
Edwards, Christofer J.;Leary, Christopher J.;Rose, Gary J.
通讯作者：
Rose, Gary J.
影响因子：
2.9
作者：
Rose, GJ;Fortune, ES
通讯作者：
Fortune, ES
影响因子：
4.5
作者：
SOEJIMA, M;NOMA, A
通讯作者：
NOMA, A
影响因子：
2.5
作者：
Kiyatkin, EA;Rebec, GV
通讯作者：
Rebec, GV