Cajal–Retzius cells in the mouse neocortex receive two types of pre‐ and postsynaptically distinct GABAergic inputs
Cajal–Retzius cells in the mouse neocortex receive two types of pre‐ and postsynaptically distinct GABAergic inputs
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小鼠新皮质中的 Cajal-Retzius 细胞接收两种类型的突触前和突触后不同的 GABA 能输入
DOI：
10.1113/jphysiol.2007.145003
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Kirischuk
中科院分区：
文献类型：
--
作者：
Knut Kirmse;A. Dvorzhak;C. Henneberger;R. Grantyn;S. Kirischuk
Cajal-Retzius (CR) 细胞是发育中的新皮质中第一层的主要细胞。它们能够产生动作电位、在第一层建立突触接触并在出生前接收兴奋性 GABA 能输入。尽管 CR 细胞参与 I 层神经元网络活动，但其突触输入的特性尚未得到表征。我们使用全细胞膜片钳技术记录了微型（mIPSC）和诱发（eIPSC）突触后电流。大多数 CR 细胞表现出两种类型的 mIPSC，即具有快速上升动力学 (mIPSCF) 和缓慢上升动力学 (mIPSCS) 的 mIPSC。 mIPSCF 的平均振幅明显大于 mIPSCS，但它们的衰减率没有差异。峰值尺度非平稳噪声分析表明，mIPSCS 和 mIPSCF 的加权单通道电导不同。此外，唑吡坦 (100 nm)（一种包含 α1 亚基的 GABAA 受体的调节剂）选择性地影响 mIPSCS，表明不同的突触后 GABAA 受体介导 mIPSCF 和 mIPSCS。 eIPSC 也分为两个具有不同上升动力学的群体。与缓慢上升的 eIPSC (eIPSCS) 相比，快速 eIPSC (eIPSCF) 表现出更高的配对脉冲比 (PPR) 和更低的 GABA 释放概率。由于 GABAB 受体拮抗剂 CGP55845 消除了观察到的 PPR 差异，IPSCF 连接处较低的释放概率可能反映了 GABAB 受体介导的 IPSCF 突触更强的强直性抑制。在低（0.1 Hz）刺激频率下，两个输入都可以有效地将突触前动作电位转换为突触后动作电位；然而，只有 IPSCF 连接才能以较高的刺激率可靠地传递突触前活动模式。因此，CR 细胞接收两个 GABA 能输入，它们的量子幅度、GABA 释放概率和信号传递的频率依赖性不同。
Cajal–Retzius (CR) cells are principal cells of layer I in the developing neocortex. They are able to generate action potentials, make synaptic contacts in layer I and receive excitatory GABAergic inputs before birth. Although CR cells participate in neuronal network activity in layer I, the properties of their synaptic inputs are not yet characterized. We recorded miniature (mIPSCs) and evoked (eIPSCs) postsynaptic currents using the whole‐cell patch‐clamp technique. Most of CR cells displayed two types of mIPSCs, namely those with fast (mIPSCF) and slow (mIPSCS) rise kinetics. The mIPSCF mean amplitude was significantly larger than that of mIPSCS, while their decay rates were not different. Peak‐scaled non‐stationary noise analysis revealed that mIPSCS and mIPSCF differed in their weighted single‐channel conductance. In addition, zolpidem (100 nm), a modulator of α1 subunit‐containing GABAA receptors, selectively affected mIPSCS suggesting that different postsynaptic GABAA receptors mediate mIPSCF and mIPSCS. eIPSCs also split into two populations with different rise kinetics. Fast eIPSCs (eIPSCF) displayed higher paired‐pulse ratio (PPR) and lower GABA release probability than slowly rising eIPSCs (eIPSCS). As CGP55845, a GABAB receptor antagonist, eliminated the observed difference in PPR, the lower release probability at IPSCF connections probably reflects a stronger tonic GABAB receptor‐mediated inhibition of IPSCF synapses. At low (0.1 Hz) stimulation frequency both inputs can effectively convert presynaptic action potentials into postsynaptic ones; however, only IPSCF connections reliably transfer the presynaptic activity patterns at higher stimulation rates. Thus, CR cells receive two GABAergic inputs, which differ in the quantal amplitude, the probability of GABA release and the frequency dependence of signal transfer.
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影响因子：
2.5
作者：
LUHMANN, HJ;PRINCE, DA
通讯作者：
PRINCE, DA
影响因子：
2.5
作者：
Zixiu Xiang;J. Huguenard;D. Prince
通讯作者：
Zixiu Xiang;J. Huguenard;D. Prince
影响因子：
2.5
作者：
Dammerman, RS;Flint, AC;Kriegstein, AR
通讯作者：
Kriegstein, AR
影响因子：
2.5
作者：
Owens, DF;Liu, XL;Kriegstein, AR
通讯作者：
Kriegstein, AR
DOI：
10.1016/0165-3806(82)90141-9
发表时间：
1982-01-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
CAVINESS, VS
通讯作者：
CAVINESS, VS