Comparison of covalently and physically cross-linked collagen hydrogels on mediating vascular network formation for engineering adipose tissue.
Comparison of covalently and physically cross-linked collagen hydrogels on mediating vascular network formation for engineering adipose tissue.
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在介导脂肪组织中介导的血管网络形成上的共价和物理交联的胶原凝胶的比较。
DOI：
10.1080/21691401.2018.1499660
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen YC
中科院分区：
文献类型：
--
作者：
Chuang CH;Lin RZ;Melero-Martin JM;Chen YC
及时的组织血管化和将工程化组织整合到患者体内在将工程化组织成功转化为临床相关疗法中起着重要作用。为了减少工程化脂肪组织血管化所需的时间，已经研究了由水凝胶提供的合适的局部微环境以支持基于细胞的功能性血管网络形成。在溶液中使用相同的生物分子可以获得两种类型的水凝胶：“物理水凝胶”，其是由于胶原蛋白链上的氨基和羧基端肽引起的热诱导的自组装原纤维起始和生长，以及“化学水凝胶”，其由水溶液中的一步酶介导诱导的侧链的共价交联产生。本文比较了不同类型胶原水凝胶、物理交联和化学交联在体内构建血管网和大尺寸血管化脂肪组织的能力。讨论了这两种水凝胶的血管网络形成与水凝胶性能之间的关系。最后，我们在化学共价交联的载有细胞的水凝胶中不存在外源性细胞因子的情况下成功地工程化血管化脂肪组织构建体（~877.6脂肪细胞/mm 2; 94%的构建体面积）。这些结果表明，操纵水凝胶的聚合方法不仅可以调节血管网络的形成，而且可以在体内再生脂肪组织。
Timely tissue vascularization and integration of engineered tissues into a patient plays an important role in the successful translation of engineered tissues into clinically relevant therapies. To decrease the time needed to vascularize an engineered adipose tissue, suitable local microenvironments provided by hydrogels to support cell-based functional vascular network formation have been investigated. Using the same biomolecule in solution two types of hydrogels can be obtained: a “physical hydrogel” which is thermal-induced self-assemble fibril initiation and growth, due to amino and carboxyl telopeptides on collagen chains, and a “chemical hydrogel” which results from the covalently cross-linking of the side chains induced by one step enzyme mediation in aqueous solution. In this paper, we compare the capability of engineering vascular network and large-sized vascularized adipose tissue in vivo in different types of collagen hydrogels, physical and chemical crosslinking. The relationships between vascular network formation and hydrogel properties for the two types of hydrogels are discussed. Finally, we successfully engineered a vascularized adipose tissue construct (~877.6 adipocytes/mm2; 94% area of a construct) in the absence of exogenous cytokines in chemical covalently crosslinking cell-laden hydrogel. These results show manipulating the polymerized methods of a hydrogel could not only modulate vascular network formation, but also regenerate adipose tissue in vivo.
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影响因子：
9.7
作者：
Chuang CH;Lin RZ;Tien HW;Chu YC;Li YC;Melero-Martin JM;Chen YC
通讯作者：
Chen YC
影响因子：
14
作者：
Lin, Ruei-Zeng;Chen, Ying-Chieh;Moreno-Luna, Rafael;Khademhosseini, Ali;Melero-Martin, Juan M.
通讯作者：
Melero-Martin, Juan M.
影响因子：
9.8
作者：
Rao, Rameshwar R.;Peterson, Alexis W.;Ceccarelli, Jacob;Putnam, Andrew J.;Stegemann, Jan P.
通讯作者：
Stegemann, Jan P.
DOI：
10.1073/pnas.1405388111
发表时间：
2014-07-15
影响因子：
11.1
作者：
Lin, Ruei-Zeng;Moreno-Luna, Rafael;Melero-Martin, Juan M.
通讯作者：
Melero-Martin, Juan M.
影响因子：
14
作者：
Helary, Christophe;Bataille, Isabelle;Giraud-Guille, Marie Madeleine
通讯作者：
Giraud-Guille, Marie Madeleine