Metabolic imaging of human cumulus cells reveals associations among metabolic profiles of cumulus cells, patient clinical factors, and oocyte maturity.
Metabolic imaging of human cumulus cells reveals associations among metabolic profiles of cumulus cells, patient clinical factors, and oocyte maturity.
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DOI：
10.1016/j.fertnstert.2021.07.1204
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发表时间：
2021-12
影响因子：
6.7
通讯作者：
Needleman DJ
中科院分区：
文献类型：
--
作者：
Venturas M;Yang X;Kumar K;Wells D;Racowsky C;Needleman DJ
目的：研究荧光寿命成像显微镜（FLIM）能否检测到积云代谢状态的差异。细胞样本，以及这种代谢状态是否与患者年龄、BMI和/或AMH以及卵母细胞成熟度有关。前瞻性观察研究。学术实验室。取卵母细胞并玻璃化后，收集卵母细胞-卵母细胞复合物中的卵丘细胞簇。用FLIM测定细胞呼吸和糖酵解所必需的内源性辅酶NAD(P)H和FAD+的自身荧光来评估CC代谢。记录患者年龄、BMI、AMH及相应卵母细胞成熟度。从FLIM获得的定量信息包括：从荧光强度获得代谢物浓度；和代谢酶接合从荧光寿命。研究了各FLIM参数与卵母细胞成熟度、患者年龄、BMI和AMH水平之间的关系。患者内部和患者之间CC群的差异。在193例患者的619个CC簇中，90个与未成熟卵母细胞相关，505个与中期卵母细胞相关。FLIM能够定量测量CC簇的代谢状态。这些参数与患者年龄和AMH显著相关，但与BMI无关。CC NAD(P)H FLIM参数和氧化还原比与封闭卵母细胞的成熟度显著相关。FLIM检测CC代谢状态的变化，显示每个患者的集群之间的差异大于患者之间的差异。FLIM可以检测CC代谢与患者年龄和AMH之间的关联，并检测成熟和未成熟卵母细胞之间的差异，表明该技术有助于识别优质卵母细胞的潜在效用。利用荧光寿命成像显微镜（FLIM）进行代谢成像，可灵敏地检测人积云细胞代谢状态的变化。这些变化与封闭卵母细胞的成熟度和患者的临床因素有关。
To study whether fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic state among cumulus. cell samples and if this metabolic state is associated with patient age, BMI and/or AMH, and maturity of the oocyte. Prospective observational study. Academic laboratory. Cumulus cell (CC) clusters from cumulus-oocyte complexes were collected after oocyte retrieval and vitrified. CC metabolism was assessed using FLIM to measure autofluorescence of NAD(P)H and FAD+, endogenous coenzymes essential for cellular respiration and glycolysis. Patient age, BMI, and AMH, and the maturity of corresponding oocytes were recorded. Quantitative information from FLIM was obtained regarding: metabolite concentrations from fluorescence intensity; and metabolite enzyme engagement from fluorescence lifetimes. Associations were investigated between each FLIM parameter and oocyte maturity, patient age, BMI and AMH level. Variance between CC clusters within and between patients. Of 619 CC clusters from 193 patients, 90 were associated with immature oocytes, 505 with metaphase II oocytes. FLIM enabled quantitative measurements of metabolic state of CC clusters. These parameters were significantly correlated with patient age and AMH, independently, but not BMI. CC NAD(P)H FLIM parameters and Redox Ratio were significantly associated with maturity of the enclosed oocyte. FLIM detects variations in metabolic state of CC, showing a greater variance among clusters from each patient than between patients. FLIM can detect CC metabolic associations with patient age and AMH and detects variations between mature and immature oocytes, suggesting the potential utility of this technique to help identify superior oocytes. Metabolic imaging using fluorescence lifetime imaging microscopy (FLIM) sensitively detects variations in metabolic state of human cumulus cells. These variations are associated with maturity of the enclosed oocyte and patient clinical factors.
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影响因子：
2.2
作者：
Heikal AA
通讯作者：
Heikal AA
影响因子：
13.3
作者：
May-Panloup, Pascale;Boucret, Lisa;Reynier, Pascal
通讯作者：
Reynier, Pascal
影响因子：
6.1
作者：
Lan, Yueyun;Zhang, Shuoping;Hu, Liang
通讯作者：
Hu, Liang
影响因子：
3.1
作者：
Lorah, Julie
通讯作者：
Lorah, Julie
影响因子：
3.6
作者：
Cinco R;Digman MA;Gratton E;Luderer U
通讯作者：
Luderer U