Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies.
Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies.
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DOI：
10.2217/bmm.10.1
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发表时间：
2010-04
影响因子：
2.2
通讯作者：
Heikal AA
中科院分区：
文献类型：
--
作者：
Heikal AA
线粒体在能量代谢、程序性细胞死亡和氧化应激中起着关键作用。病变细胞中突变的线粒体DNA损害了关键酶复合物的结构，因此损害了线粒体功能，这导致了无数与健康相关的疾病，如癌症、神经退行性疾病、糖尿病和衰老。线粒体和代谢异常的早期检测是有效诊断和治疗干预的重要步骤。还原型烟酰胺腺嘌呤二核苷酸（NADH）和黄素腺嘌呤二核苷酸（FAD）在多种细胞氧化还原反应中起重要作用。重要的是，NADH和FAD是天然的荧光，这允许活细胞和组织的代谢活动的非侵入性成像。此外，NADH和FAD自发荧光可以使用不同的波长激发，用于互补成像方法，并且对蛋白质结合和局部环境敏感。本文重点介绍了最近的发展有关细胞内的NADH和FAD作为潜在的生物标志物的代谢和线粒体活动。
Mitochondria play a pivotal role in energy metabolism, programmed cell death and oxidative stress. Mutated mitochondrial DNA in diseased cells compromises the structure of key enzyme complexes and, therefore, mitochondrial function, which leads to a myriad of health-related conditions such as cancer, neurodegenerative diseases, diabetes and aging. Early detection of mitochondrial and metabolic anomalies is an essential step towards effective diagnoses and therapeutic intervention. Reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) play important roles in a wide range of cellular oxidation–reduction reactions. Importantly, NADH and FAD are naturally fluorescent, which allows noninvasive imaging of metabolic activities of living cells and tissues. Furthermore, NADH and FAD autofluorescence, which can be excited using distinct wavelengths for complementary imaging methods and is sensitive to protein binding and local environment. This article highlights recent developments concerning intracellular NADH and FAD as potential biomarkers for metabolic and mitochondrial activities.
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影响因子：
5.3
作者：
Brecht, M;Fee, MS;Osten, P
通讯作者：
Osten, P
影响因子：
11.2
作者：
Bird, DK;Yan, L;Ramanujam, N
通讯作者：
Ramanujam, N
影响因子：
56.9
作者：
CHANCE, B;JOBSIS, F;COHEN, P
通讯作者：
COHEN, P
影响因子：
56.9
作者：
CHANCE, B
通讯作者：
CHANCE, B
影响因子：
3.2
作者：
BENSON, RC;MEYER, RA;MCKHANN, GM
通讯作者：
MCKHANN, GM