Association between single nucleotide variants and severe chronic pain in older adult patients after lower extremity arthroplasty.
Association between single nucleotide variants and severe chronic pain in older adult patients after lower extremity arthroplasty.
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DOI：
10.1186/s13018-023-03683-y
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发表时间：
2023-03-09
影响因子：
2.6
通讯作者：
Ma, Zhengliang
中科院分区：
文献类型：
--
作者：
Xu, Rui;Jin, Yinan;Tang, Suhong;Wang, Wenwen;Sun, Yu-E;Liu, Yue;Zhang, Wei;Hou, Bailing;Huang, Yulin;Ma, Zhengliang
髋关节或膝关节骨关节炎（OA）是世界范围内致残的主要原因之一，主要发生在老年人中。全髋或全膝关节置换术是治疗OA最有效的方法。然而，严重的术后疼痛导致预后不良。因此，研究老年人下肢关节置换术后严重慢性疼痛的群体遗传学和相关基因有助于提高治疗质量。我们收集了2020年9月至2021年2月在南京大学医学院附属鼓楼医院接受下肢关节置换术的老年患者的血液样本。入组患者在术后第90天使用数字评定量表提供疼痛强度的测量值。采用数字化评分法将患者分为病例组（A组）和对照组（B组）各10例。从两组的血液样品中分离DNA用于全外显子组测序。总共在507个基因区域中鉴定出661个变异体，这些变异体在两组之间存在显著差异（P <0.05），包括CASP 5、RASGEF1A、CYP4B1等，这些基因主要参与生物学过程，包括细胞与细胞粘附、ECM与受体相互作用、代谢、生物活性物质的分泌、离子结合和转运、DNA甲基化的调节、和染色质组装。目前的研究表明，基因内的一些变异与下肢关节成形术后老年患者的严重术后慢性疼痛显著相关，表明慢性术后疼痛的遗传易感性。该研究根据ICMJE指南注册。试验注册号为ChiCTR 2000031655，注册日期为2020年4月6日。在线版本包含补充材料，可通过10.1186/s13018 - 023 - 03683-y获得。
Hip or knee osteoarthritis (OA) is one of the main causes of disability worldwide and occurs mostly in the older adults. Total hip or knee arthroplasty is the most effective method to treat OA. However, severe postsurgical pain leading to a poor prognosis. So, investigating the population genetics and genes related to severe chronic pain in older adult patients after lower extremity arthroplasty is helpful to improve the quality of treatment. We collected blood samples from elderly patients who underwent lower extremity arthroplasty from September 2020 to February 2021 at the Drum Tower Hospital Affiliated to Nanjing University Medical School. The enrolled patients provided measures of pain intensity using the numerical rating scale on the 90th day after surgery. Patients were divided into the case group (Group A) and the control group (Group B) including 10 patients respectively by the numerical rating scale. DNA was isolated from the blood samples of the two groups for whole-exome sequencing. In total, 661 variants were identified in the 507 gene regions that were significantly different between both groups (P < 0.05), including CASP5, RASGEF1A, CYP4B1, etc. These genes are mainly involved in biological processes, including cell–cell adhesion, ECM–receptor interaction, metabolism, secretion of bioactive substances, ion binding and transport, regulation of DNA methylation, and chromatin assembly. The current study shows some variants within genes are significantly associated with severe postsurgical chronic pain in older adult patients after lower extremity arthroplasty, indicating a genetic predisposition for chronic postsurgical pain. The study was registered according to ICMJE guidelines. The trial registration number is ChiCTR2000031655 and registration date is April 6th, 2020. The online version contains supplementary material available at 10.1186/s13018-023-03683-y.
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影响因子：
7.3
作者：
Hasegawa M;Yoshida T;Sudo A
通讯作者：
Sudo A
影响因子：
1.6
作者：
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3.2
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通讯作者：
Sharkey, Peter F.
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4
作者：
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通讯作者：
Wang, Qiang