Formation of a mast cell synapse: Fc epsilon RI membrane dynamics upon binding mobile or immobilized ligands on surfaces.
Formation of a mast cell synapse: Fc epsilon RI membrane dynamics upon binding mobile or immobilized ligands on surfaces.
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DOI：
10.4049/jimmunol.0903071
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发表时间：
2010-02-01
期刊：
影响因子：
--
通讯作者：
Timlin JA
中科院分区：
文献类型：
--
作者：
Carroll-Portillo A;Spendier K;Pfeiffer J;Griffiths G;Li H;Lidke KA;Oliver JM;Lidke DS;Thomas JL;Wilson BS;Timlin JA
肥大细胞上的高亲和力IgE受体（FcεRI）在与移动的双层结合抗原呈递时形成“突触”。在这里，我们表明，受体重组内接触肥大细胞膜是显着不同的结合后，移动的和固定的配体。用荧光抗DNP IgE引发的大鼠嗜碱性白血病肥大细胞（RBL-2 H3）被呈现双层掺入的单价DNP-脂质（移动的配体）或化学交联的多价DNP（固定化配体）的表面接合。全内反射荧光成像和电子显微镜方法被用来可视化受体重组的接触网站。同时分析了FcεRI与突触处其他细胞成分（如肌动蛋白、胆固醇和LAT）的空间关系。用固定化多价配体刺激肥大细胞导致典型水平的脱粒。值得注意的是，脱粒也遵循肥大细胞与呈现移动的单价配体的双层的相互作用。与移动的配体接合的受体聚结成大的富含胆固醇的簇，其占据接触膜的中心部分。这些数据表明FcεRI交联不是触发肥大细胞信号传导的必要步骤，并表明移动的受体的密集群体能够在配体识别后启动低水平脱粒。
High affinity IgE receptors (FcεRI) on mast cells form a “synapse” when presented with mobile, bilayer incorporated antigen. Here, we show that receptor reorganization within the contacting mast cell membrane is markedly different upon binding of mobile and immobilized ligands. Rat basophilic leukemia mast cells (RBL-2H3) primed with fluorescent anti-DNP IgE were engaged by surfaces presenting either bilayer-incorporated, monovalent DNP-lipid (mobile ligand) or chemically crosslinked, multivalent DNP (immobilized ligand). Total internal reflection fluorescence imaging and electron microscopy methods were used to visualize receptor reorganization at the contact site. The spatial relationships of FcεRI to other cellular components at the synapse, such as actin, cholesterol and LAT, were also analyzed. Stimulation of mast cells with immobilized polyvalent ligand resulted in typical levels of degranulation. Remarkably, degranulation also followed interaction of mast cells with bilayers presenting mobile, monovalent ligand. Receptors engaged with mobile ligand coalesce into large, cholesterol-rich clusters that occupy the central portion of the contacting membrane. These data indicate that FcεRI crosslinking is not an obligatory step in triggering mast cell signaling and suggest that dense populations of mobile receptors are capable of initiating low level degranulation upon ligand recognition.
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影响因子：
32.4
作者：
Andrews, Nicholas L.;Pfeiffer, Janet R.;Lidke, Diane S.
通讯作者：
Lidke, Diane S.
影响因子：
7.3
作者：
Depoil, David;Weber, Michele;Batista, Facundo D.
通讯作者：
Batista, Facundo D.
影响因子：
3.6
作者：
Grodzki, Ana Cristina G.;Moon, Kyungduk D.;Siraganian, Reuben P.
通讯作者：
Siraganian, Reuben P.
DOI：
10.1073/pnas.0710258105
发表时间：
2007-12-18
影响因子：
11.1
作者：
Kaizuka, Yoshihisa;Douglass, Adam D.;Vale, Ronald D.
通讯作者：
Vale, Ronald D.
影响因子：
56.9
作者：
Grakoui, A;Bromley, SK;Dustin, ML
通讯作者：
Dustin, ML