Transcriptome analysis identifies signaling pathways related to meat quality in broiler chickens - the extracellular matrix (ECM) receptor interaction signaling pathway.
Transcriptome analysis identifies signaling pathways related to meat quality in broiler chickens - the extracellular matrix (ECM) receptor interaction signaling pathway.
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转录组分析确定了与肉鸡肉质相关的信号通路——细胞外基质（ECM）受体相互作用信号通路。
DOI：
10.1016/j.psj.2021.101135
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发表时间：
2021-06
期刊：
影响因子：
4.4
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
San J;Du Y;Wu G;Xu R;Yang J;Hu J
肉质特性，包括多汁性、风味和嫩度，主要归因于肌肉总脂肪含量、肌内脂肪（IMF）及其脂肪酸组成，这些受脂质摄取、运输、合成以及后续代谢之间的平衡所调控，涉及许多基因和途径。然而，详细的分子机制仍不清楚。本研究的目的是确定与鸡肉品质相关的关键信号通路，并为改善鸡肉品质提供帮助。本研究报告了庄河大骨鸡和爱拔益加肉鸡（AA鸡）的胸肌和腿肌的RNA测序分析。我们鉴定出了某些影响IMF沉积的差异表达基因，例如在庄河大骨鸡中上调的EHHADH、TECRL、NDUFAB1、PCCB和HIBCH，以及在AA鸡中上调的GCDH、TPI1、ABHD13、PSMC1、MYST2和FBXO11。利用京都基因与基因组百科全书进行的通路分析表明，细胞外基质（ECM） - 受体相互作用通路在两种组织中共同富集，并形成其他富集通路的一个子通路。有趣的是，ECM - 受体相互作用通路基因在不同的基因库中受到不同的调控。胶原蛋白作为主要的ECM成分，以及层粘连蛋白和整合素β1跨膜受体在AA鸡的两种组织中均显著下调。结果表明，ECM - 受体相互作用通路通过影响肌内脂肪细胞的代谢来影响鸡肉品质。对该信号通路的进一步研究将有助于鸡肉品质的改善。
Meat quality characteristics, including juiciness, flavor, and tenderness, can be mostly attributed to the total muscle fat content, intramuscular fat (IMF), and the composition of its fatty acids, which are regulated by the balance between lipid uptake, transport, synthesis, and subsequent metabolism, involving many genes and pathways. However, the detailed molecular mechanisms remain unclear. The purpose of this study was to identify the key signaling pathways related to chicken meat quality, and to provide help for improving chicken meat quality. The present study reports the RNA-sequencing analysis of pectorales and crureus of the Zhuanghe dagu chicken and the Arbor Acres Broiler chicken (AA chicken). We identified certain differentially expressed genes that affect IMF deposition, such as EHHADH, TECRL, NDUFAB1, PCCB, and HIBCH, which were upregulated in Zhuanghe dagu chicken , and GCDH, TPI1, ABHD13, PSMC1, MYST2, and FBXO11, which were upregulated in AA chickens. Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes indicated that the extracellular matrix (ECM)–receptor interaction pathway is co-enriched in both tissues, and forms a sub-pathway of other enriched pathways. Intriguingly, the ECM–receptor interaction pathway genes are regulated differently in different gene pools. Collagens, which are main ECM constituents, and laminin and integrin β1 transmembrane receptors were significantly downregulated in both tissues of the AA chicken. The results showed that the ECM-receptor interaction pathway affect the quality of chicken meat by affecting the metabolism of intramuscular adipocytes. Further investigation of this signaling pathway will be helpful to the improvement of chicken meat quality.
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DOI：
10.1080/23802359.2020.1785351
发表时间：
2020-07-02
期刊：
Mitochondrial DNA. Part B, Resources
影响因子：
--
作者：
Gu J;Li S
通讯作者：
Li S
影响因子：
4.4
作者：
Dransfield, E;Sosnicki, AA
通讯作者：
Sosnicki, AA
影响因子：
29
作者：
Liu, J;DeYoung, SM;Saltiel, AR
通讯作者：
Saltiel, AR
影响因子：
7.1
作者：
Calkins, C. R.;Hodgen, J. M.
通讯作者：
Hodgen, J. M.
影响因子：
2.7
作者：
Al-Nasser, A.;Al-Khalaifah, H.;Al-Mansour, H.
通讯作者：
Al-Mansour, H.