Architecture-Guided Fluid Flow Directs Renal Biomineralization.
Architecture-Guided Fluid Flow Directs Renal Biomineralization.
复制标题
DOI：
10.1038/s41598-018-30717-x
复制
发表时间：
2018-09-21
影响因子：
4.6
通讯作者：
Stoller ML
中科院分区：
文献类型：
--
作者：
Ho SP;Chen L;Allen FI;Hsi RS;Shimotake AR;Wiener SV;Kang M;Minor AM;Stoller ML
肾钙质沉着症通常始于髓乳头复合体 (MPC) 尖端的磷酸钙沉积，称为兰德尔斑 (RP)。结合 MPC 内近端观察到的生物矿物质，我们假设存在一种机械生物学开关，可以在远离管内生物矿物质的 MPC 尖端触发间质生物矿化。人类 MPC 的微型 X 射线计算机断层扫描与透射和扫描电子显微照片相关，X 射线能量色散光谱法证明了有关解剖学特异性生物矿物质的新发现。丰富的近端肾小管内生物矿物质与远端间质性 RP 的出现有关。 MPC 的基本结构以及 MPC 近端和远端位置的矿物密度显着不同。观察到在近管内位置的球状矿物内主要是板状矿物或径向取向的板状微晶，在远端间隙位置的球状矿物内观察到核壳型微晶。根据结构特异性生物矿物质的 MPC 解剖位置，假设在近端位置和远端位置之间发生无矿物质区内的生物转换。 “开”和“关”开关取决于小管直径变化引起的压差变化； “文丘里效应”改变“周向应变”，并最终导致远端肾小管壁间质晶体沉积，以响应近端肾小管阻塞。这些远端间质矿化可进入肾脏集合系统，将肾钙质沉着症与肾结石联系起来。
Nephrocalcinosis often begins on a calcium phosphate deposit, at the tip of the medullo-papillary complex (MPC) known as Randall’s plaque (RP). Contextualizing proximally observed biominerals within the MPC has led us to postulate a mechanobiological switch that can trigger interstitial biomineralization at the MPC tip, remote from the intratubular biominerals. Micro X-ray computed tomography scans of human MPCs correlated with transmission and scanning electron micrographs, and X-ray energy dispersive spectrometry demonstrated novel findings about anatomically-specific biominerals. An abundance of proximal intratubular biominerals were associated with emergence of distal interstitial RP. The fundamental architecture of the MPC and mineral densities at the proximal and distal locations of the MPC differed markedly. A predominance of plate-like minerals or radially oriented plate-like crystallites within spheroidal minerals in the proximal intratubular locations, and core-shell type crystallites within spheroidal minerals in distal interstitial locations were observed. Based on the MPC anatomic location of structure-specific biominerals, a biological switch within the mineral-free zone occurring between the proximal and distal locations is postulated. The “on” and “off” switch is dependent on changes in the pressure differential resulting from changes in tubule diameters; the “Venturi effect” changes the “circumferential strain” and culminates in interstitial crystal deposits in the distal tubule wall in response to proximal tubular obstruction. These distal interstitial mineralizations can emerge into the collecting system of the kidney linking nephrocalcinosis with nephrolithiasis.
登录
查看更多内容
影响因子：
15.6
作者：
KAHLWEIT, M
通讯作者：
KAHLWEIT, M
影响因子：
15.3
作者：
OLIVER, J;MACDOWELL, M
通讯作者：
MACDOWELL, M
影响因子：
15.3
作者：
OLIVER, J;MACDOWELL, M;LEE, YC
通讯作者：
LEE, YC
DOI：
10.1152/ajprenal.00398.2003
发表时间：
2004-10-01
影响因子：
4.2
作者：
Layton, AT;Pannabecker, TL;Layton, HE
通讯作者：
Layton, HE
影响因子：
15.9
作者：
Evan, AP;Lingeman, JE;Grynpas, M
通讯作者：
Grynpas, M