Segregation of myoblast fusion and muscle-specific gene expression by distinct ligand-dependent inactivation of GSK-3β.
Segregation of myoblast fusion and muscle-specific gene expression by distinct ligand-dependent inactivation of GSK-3β.
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DOI：
10.1007/s00018-010-0467-7
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发表时间：
2011-02
影响因子：
8
通讯作者：
Langen, R. C. J.
中科院分区：
文献类型：
--
作者：
Pansters, N. A. M.;van der Velden, J. L. J.;Kelders, M. C. J. M.;Laeremans, H.;Schols, A. M. W. J.;Langen, R. C. J.
成肌分化涉及成肌细胞融合和肌肉特异性基因表达的诱导，这两者都受到药理学（LiCl）、遗传学或IGF-I介导的GSK-3β失活的刺激。为了评估肌源性分化的刺激是否与配体介导的GSK-3β失活共同，研究了响应Wnt-3a的成肌细胞融合和肌肉特异性基因表达。此外，评估了IGF-I/GSK-3β/NFATc 3和Wnt/GSK-3β/β-连环蛋白信号传导之间的串扰。虽然Wnt-3a和LiCl都促进成肌细胞融合，但LiCl增加了肌肉特异性基因表达，而Wnt-3a或β-连环蛋白过表达则没有增加。此外，LiCl和IGF-I，而不是Wnt-3a，增加NFATc 3转录活性。相比之下，Wnt-3a和LiCl增加了β-连环蛋白依赖的转录活性，但IGF-I没有增加。这些结果首次揭示了在响应GSK-3β失活的不同配体特异性信号途径刺激成肌细胞分化后，成肌细胞融合和肌肉特异性基因表达的分离调节。本文的在线版本（doi：10.1007/s 00018 -010-0467-7）包含补充材料，可供授权用户使用。
Myogenic differentiation involves myoblast fusion and induction of muscle-specific gene expression, which are both stimulated by pharmacological (LiCl), genetic, or IGF-I-mediated GSK-3β inactivation. To assess whether stimulation of myogenic differentiation is common to ligand-mediated GSK-3β inactivation, myoblast fusion and muscle-specific gene expression were investigated in response to Wnt-3a. Moreover, crosstalk between IGF-I/GSK-3β/NFATc3 and Wnt/GSK-3β/β-catenin signaling was assessed. While both Wnt-3a and LiCl promoted myoblast fusion, muscle-specific gene expression was increased by LiCl, but not by Wnt-3a or β-catenin over-expression. Furthermore, LiCl and IGF-I, but not Wnt-3a, increased NFATc3 transcriptional activity. In contrast, β-catenin-dependent transcriptional activity was increased by Wnt-3a and LiCl, but not IGF-I. These results for the first time reveal a segregated regulation of myoblast fusion and muscle-specific gene expression following stimulation of myogenic differentiation in response to distinct ligand-specific signaling routes of GSK-3β inactivation. The online version of this article (doi:10.1007/s00018-010-0467-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1083/jcb.148.4.691
发表时间：
2000-02-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Farr GH 3rd;Ferkey DM;Yost C;Pierce SB;Weaver C;Kimelman D
通讯作者：
Kimelman D
影响因子：
--
作者：
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通讯作者：
Yu, B.
影响因子：
4.8
作者：
Ding, VW;Chen, RH;McCormick, F
通讯作者：
McCormick, F
影响因子：
4
作者：
Huelsken, J;Behrens, J
通讯作者：
Behrens, J
影响因子：
3.6
作者：
Descamps, Simon;Arzouk, Hayat;Levin, Jonathan
通讯作者：
Levin, Jonathan