A rapid shift to high-grain diet results in dynamic changes in rumen epimural microbiome in sheep.
A rapid shift to high-grain diet results in dynamic changes in rumen epimural microbiome in sheep.
复制标题
向高谷物饮食的快速转变导致绵羊瘤胃外微生物组的动态变化。
DOI：
10.1017/s1751731118003269
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发表时间：
2019-08
期刊：
影响因子：
3.6
通讯作者：
Mao S Y
中科院分区：
文献类型：
--
作者：
Seddik H;Xu L;Wang Y;Mao S Y
反刍动物快速转向高谷物（HG）日粮会影响瘤胃上皮的功能，但绵羊瘤胃上皮相关细菌群落组成的动态变化仍需进一步研究。20只公羔羊随机分为4组（n = 5）。第一组动物接受干草饲料，并代表对照组（CON）。同时，其他三组（HG组）中的动物迅速转换为HG饮食（60%浓缩物），分别持续7天（HG 7）、14天（HG 14）和28天（HG 28）。结果表明，由于快速转换为HG日粮，瘤胃pH显著降低（P <0.001），而丁酸（P <0.001）、乳酸（P = 0.001）、戊酸（P = 0.008）和总挥发性脂肪酸（P = 0.001）浓度升高。多样性估计值显示出显着下降后的转变没有恢复HG喂养继续。主坐标分析显示CON组与所有HG组单独聚类，仅HG 7和HG 28之间存在显著差异（P = 0.034）。非参数多变量分析（npmv R-package）推断，通过未观察状态重建（PICRUSt）预测的京都基因和基因组百科全书（KEGG），在门和群落系统发育研究中的主要显著差异主要归因于饮食组成与施用期（P = 0.140，0.545）相比，施用期（P <0.001，P = 0.001）仅对属（P = 0.001）和操作分类单位（OTUs）水平（P = 0.011）表现出显著影响。Kruskal-Wallis检验推断，由于饮食组成的变化，六门显示出显着的效果。在属的水平上，HG喂养改变了丰富的12个类群，其中4个表现出显着的变化，由于HG饮食应用的持续时间。类似地，我们发现21个OTU由于HG饮食应用的持续时间而显示出显著变化。此外，PICRUSt预测的基因丰度显示，HG喂养显著影响KEGG中鉴定的7条代谢途径。其中，高糖组与碳水化合物代谢相关基因家族的丰度显著高于高糖组（P = 0.027）。总的来说，这些结果表明，快速过渡到HG饮食导致绵羊瘤胃发酵以及瘤胃上皮相关微生物组的组成和功能发生显著变化，而HG饮食应用的持续时间导致一些物种的丰度发生急剧变化。
The rapid shift to high-grain (HG) diets in ruminants can affect the function of the rumen epithelium, but the dynamic changes in the composition of the epithelium-associated (epimural) bacterial community in sheep still needs further investigation. Twenty male lambs were randomly allocated to four groups (n = 5). Animals of the first group received hay diet and represented a control group (CON). Simultaneously, animals in the other three groups (HG groups) were rapidly shifted to an HG diet (60% concentrate)which continued for 7 (HG7), 14 (HG14) and 28 (HG28) days, correspondingly. Results showed that ruminal pH dramatically decreased due to the rapid shift to the HG diet (P <0.001), while, the concentrations of butyrate (P <0.001), lactate (P = 0.001), valerate (P = 0.008) and total volatile fatty acids (P = 0.001) increased. Diversity estimators showed a dramatic decrease after the shift without recovering as the HG feeding continued. The principal coordinates analysis showed that CON group clustered separately from all HG groups with the presence of significant difference only between HG7 and HG28 (P = 0.034). The non-parametric multivariate analysis (npmv R-package) deduced that the primary significant differences in phyla and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt)-predicted Kyoto Encyclopedia of Genes and Genomes (KEGGs) was attributed mainly to the diet composition (P <0.001, P = 0.001) compared to its application period (P = 0.140, 0.545) which showed a significant effect only on the genus (P = 0.001) and the operational taxonomic units (OTUs) level (P = 0.011). The Kruskal-Wallis test deduced that six phyla showed a significant effect due to the shift in diet composition. At the genus level, HG feeding altered the abundance of 12 taxa, four of which showed a significant variation due to the duration of the HG diet application. Similarly, we found that 21 OTUs showed significant variations due to the duration of the HG diet application. Furthermore, the genes abundance predicted by PICRUSt revealed that the HG feeding significantly affected seven metabolic pathways identified in the KEGG. Particularly, the abundance of gene families associated with carbohydrates metabolism were significantly higher in HG feeding groups (P = 0.027). Collectively, these results revealed that the rapid transition to an HG diet causes dramatic alterations in ruminal fermentation and the composition and function of ruminal epithelium-associated microbiome in sheep, while, the duration of the HG diet application causes drastic alterations to the abundance of some species.
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影响因子：
4.6
作者：
Kelly WJ;Cookson AL;Altermann E;Lambie SC;Perry R;Teh KH;Otter DE;Shapiro N;Woyke T;Leahy SC
通讯作者：
Leahy SC
影响因子：
29.4
作者：
Y. Ni;Huey‐Huey Chua;H. Chou
通讯作者：
Y. Ni;Huey‐Huey Chua;H. Chou
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
2.4
作者：
D. Stewart
通讯作者：
D. Stewart
影响因子：
3.5
作者：
Gao, X.;Oba, M.
通讯作者：
Oba, M.