Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna.
Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna.
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不同区域细胞的比较转录组分析揭示了羊肚菌菌核起始阶段的ROS反应机制
DOI：
10.1038/s41598-021-87784-w
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发表时间：
2021-05-03
影响因子：
4.6
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Liu Q;He G;Wei J;Dong C
羊肚菌是一些最珍贵的食用和药用蘑菇，具有巨大的经济和科学价值。近年来，我国大规模地实现了露地栽培。羊肚菌菌核的形成是羊肚菌生命周期中最重要的阶段之一，以前的研究表明活性氧（ROS）在其中起着重要的作用。然而，在菌核起始（SI）阶段的ROS反应机制知之甚少。在这项研究中，比较转录组分析与菌核和菌丝细胞在同一平板上的不同区域在SI阶段。S1期菌核形成区和菌丝生长区基因表达差异显著。GO和KEGG分析表明，菌丝区的代谢特征更为活跃，而转录过程、DNA修复和蛋白质加工则集中在菌核细胞中。H2 O2产生相关基因在菌丝区表达量高，而H2 O2清除相关基因在菌核细胞中表达量高，导致菌丝区H2 O2浓度高于菌核。H2 O2诱导的信号通路在菌核和菌丝细胞的基因表达中观察到微小的差异;然而，在H2 O2诱导的信号通路中重要的转录因子MSN 2的靶基因的表达水平显著不同。MSN 2通过调节这些靶基因增强了菌核中的胁迫反应调节。小分子热休克蛋白也被发现在菌核细胞上调。本研究表明，菌核细胞比菌丝细胞更能抵抗活性氧胁迫，这是通过相关基因的转录调控实现的。
Morels are some of the most highly prized edible and medicinal mushrooms, with great economic and scientific value. Outdoor cultivation has been achieved and expanded on a large scale in China in recent years. Sclerotial formation is one of the most important phases during the morel life cycle, and previous reports indicated that reactive oxygen species (ROS) play an important role. However, ROS response mechanisms at sclerotial initiation (SI) stage are poorly understood. In this study, comparative transcriptome analyses were performed with sclerotial and hyphal cells at different areas in the same plate at SI stage. Gene expression was significantly different at SI stage between sclerotial formation and mycelia growth areas. GO and KEGG analyses indicated more vigorous metabolic characteristics in the hyphae area, while transcription process, DNA repair, and protein processing were enriched in sclerotial cells. Gene expression related to H2O2 production was high in the hyphae area, while expression of H2O2-scavenging genes was high in sclerotial cells, leading to a higher H2O2 concentration in the hyphal region than in the sclerotium. Minor differences were observed in gene expression of H2O2-induced signaling pathway in sclerotial and hyphal cells; however, expression levels of the target genes of transcription factor MSN2, important in the H2O2-induced signaling pathways, were significantly different. MSN2 enhanced stress response regulation in sclerotia by regulating these target genes. Small molecular HSPs were also found upregulated in sclerotial cells. This study indicated that sclerotial cells are more resistant to ROS stress than hyphal cells through transcriptional regulation of related genes.
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影响因子：
5
作者：
Liu, Qizheng;Zhao, Zehui;Dong, Caihong
通讯作者：
Dong, Caihong
影响因子：
3.8
作者：
Howard MJ;Wilson SH
通讯作者：
Wilson SH
影响因子：
64.5
作者：
Glover, JR;Lindquist, S
通讯作者：
Lindquist, S
影响因子：
3.5
作者：
Gaur R;Tiwari S
通讯作者：
Tiwari S
影响因子：
2.6
作者：
Alugoju, Phaniendra;Janardhanshetty, Sudharshan Setra;Dyavaiah, Madhu
通讯作者：
Dyavaiah, Madhu