The synovial proteome: analysis of fibroblast-like synoviocytes.
The synovial proteome: analysis of fibroblast-like synoviocytes.
复制标题
DOI：
10.1186/ar1153
复制
发表时间：
2004
影响因子：
4.9
通讯作者：
Wilkins JA
中科院分区：
文献类型：
--
作者：
Dasuri K;Antonovici M;Chen K;Wong K;Standing K;Ens W;El-Gabalawy H;Wilkins JA
本研究旨在确定类风湿关节炎患者滑膜成纤维细胞样细胞的蛋白表达模式。用双向聚丙烯酰胺凝胶电泳法分离细胞蛋白质，用基质辅助激光解吸电离质谱仪分析凝胶内消化的蛋白质。共检查了368个地点，进行了254次鉴定。这些研究确定了一些与正常或病理性FLS功能有关的蛋白质(例如尿苷二磷葡萄糖脱氢酶、Galectin 1和Galectin 3)，或者被表征为类风湿关节炎潜在自身抗原的蛋白质(例如Bip、Coligin、HC GP-39)。19号染色体开放阅读框10的一个新的未知蛋白质产物也被检测到作为FLS细胞的一个明显的主要成分。这些结果证明了高含量蛋白质组学方法在FLS组成分析中的应用。
The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial (FLS) cells derived from the synovia of rheumatoid arthritis patients. The cellular proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the in-gel digested proteins were analyzed by matrix-assisted laser desorption ionization mass spectrometry. A total of 368 spots were examined and 254 identifications were made. The studies identified a number of proteins that have been implicated in the normal or pathological FLS function (e.g. uridine diphosphoglucose dehydrogenase, galectin 1 and galectin 3) or that have been characterized as potential autoantigens in rheumatoid arthritis (e.g. BiP, colligin, HC gp-39). A novel uncharacterized protein product of chromosome 19 open reading frame 10 was also detected as an apparently major component of FLS cells. These results demonstrate the utility of high-content proteomic approaches in the analysis of FLS composition.
登录
查看更多内容
影响因子：
32.4
作者：
Pflanz, S;Timans, JC;Kastelein, RA
通讯作者：
Kastelein, RA
影响因子：
--
作者：
Ohshima, S;Kuchen, S;Neidhart, M
通讯作者：
Neidhart, M
影响因子：
--
作者：
Neidhart, M;Seemayer, CA;Gay, S
通讯作者：
Gay, S
影响因子：
4.4
作者：
Corrigall, VM;Bodman-Smith, MD;Panayi, GS
通讯作者：
Panayi, GS
DOI：
10.1074/mcp.m300048-mcp200
发表时间：
2003-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Krokhin O;Li Y;Andonov A;Feldmann H;Flick R;Jones S;Stroeher U;Bastien N;Dasuri KV;Cheng K;Simonsen JN;Perreault H;Wilkins J;Ens W;Plummer F;Standing KG
通讯作者：
Standing KG