Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs.
Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs.
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DOI：
10.1016/j.actbio.2013.12.026
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发表时间：
2014-05
影响因子：
9.7
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Levorson, Erica J.;Santoro, Marco;Kasper, F. Kurtis;Mikos, Antonios G.
在这项工作中，研究了间充质干细胞（MSCs）和软骨细胞共培养中直接细胞-细胞接触对改善非织造纤维聚（-内酯）（PCL）支架内软骨样细胞外基质（ECM）沉积的影响。为此，软骨细胞和间充质干细胞要么通过在单个PCL支架上混合直接接触共培养，要么通过间接共培养，将两种细胞类型播种在单独的支架上，然后在同一系统中静态或在生物反应器中介质灌注下共同培养。在静态培养中，间接共培养组的软骨细胞支架产生的糖胺聚糖和胶原蛋白的数量明显高于最初播种相同数量软骨细胞的直接共培养组。此外，在静态培养中，改善的ECM产生与更大的细胞增殖和整个支架的分布有关。在灌注培养中，流量对软骨细胞的增殖有显著影响。间接共培养组含软骨细胞支架内的ECM含量接近或超过直接共培养组。此外，在生物反应器培养中，有迹象表明软骨细胞对间充质干细胞的软骨形成有影响，软骨ECM合成能力的增加证明了这一点。这项工作表明，利用两种不同细胞类型（软骨细胞和间充质干细胞）分泌的因子，即使在没有近胞嘧啶信号传导的情况下，也有可能产生用于软骨再生的PCL/ECM杂交支架。
In this work, the influence of direct cell-cell contact in co-cultures of mesenchymal stem cells (MSCs) and chondrocytes for the improved deposition of cartilage-like extracellular matrix (ECM) within nonwoven fibrous poly(∊ -caprolactone) (PCL) scaffolds was examined. To this end, chondrocytes and MSCs were either co-cultured in direct contact by mixing on a single PCL scaffold or via indirect co-culture whereby the two cell types were seeded on separate scaffolds which were then cultured together in the same system either statically or under media perfusion in a bioreactor. In static cultures, the chondrocyte scaffold of an indirectly co-cultured group generated significantly greater amounts of glycosaminoglycan and collagen than the direct co-culture group initially seeded with the same number of chondrocytes. Furthermore, improved ECM production was linked to greater cellular proliferation and distribution throughout the scaffold in static culture. In perfusion cultures, flow had a significant effect on the proliferation of the chondrocytes. The ECM contents within the chondrocyte containing scaffolds of the indirect co-culture groups either approximated or surpassed the amounts generated within the direct co-culture group. Additionally, within bioreactor culture there were indications that chondrocytes had an influence on the chondrogenesis of MSCs as evidenced by increases in cartilaginous ECM synthetic capacity. This work demonstrates that it is possible to generate PCL/ECM hybrid scaffolds for cartilage regeneration by utilizing the factors secreted by two different cell types, chondrocytes and MSCs, even in the absence of juxtacrine signaling.
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影响因子：
--
作者：
Barnes, Catherine P.;Pemble, Charles W.;Bowlin, Gary L.
通讯作者：
Bowlin, Gary L.
影响因子：
--
作者：
Gomes, ME;Bossano, CM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
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作者：
Chen, Wei-Hong;Lai, Ming-Tang;Deng, Win-Ping
通讯作者：
Deng, Win-Ping
影响因子：
5.6
作者：
Hwang, Nathaniel S.;Varghese, Shyni;Elisseeff, Jennifer
通讯作者：
Elisseeff, Jennifer
影响因子：
4.1
作者：
Huey, Daniel J.;Sanchez-Adams, Johannah;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.