Monoclonal antibodies inhibitory to human plasmin. Definitive demonstration of a role for plasmin in activating the proenzyme of urokinase-type plasminogen activator.
Monoclonal antibodies inhibitory to human plasmin. Definitive demonstration of a role for plasmin in activating the proenzyme of urokinase-type plasminogen activator.
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抑制人纤溶酶的单克隆抗体。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
R. Stephens
中科院分区：
文献类型：
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作者：
P. Sim;D. Fayle;W. Doe;R. Stephens
针对纯化的人纤溶酶原提出的四种单克隆抗体，其特征在于它们对纤溶酶原的活化和纤溶酶的三种酶性质的影响：（a）硫代酯裂解，（B）纤维蛋白溶解，（c）高Mr尿激酶转化为低Mr形式。没有单克隆抗体抑制尿激酶激活纤溶酶原（plg）。本研究中表征的单克隆抗体分为三组。抗-plg 1抑制（a）、（B）和（c），而抗-plg 2在不同程度上抑制活性（a）、（B）和（c），但也与纤溶酶形成对十二烷基硫酸钠稳定的复合物。抗-plg 3和抗-plg 4仅抑制活性（c）。这些单克隆抗体的选择性使用明确地表明，纤溶酶介导尿激酶型纤溶酶原激活剂的酶原形式的激活。因此，除了它们在亲和层析中的用途之外，这些抗体对于确定纤溶酶在细胞外基质降解机制中的作用是有价值的。
Four monoclonal antibodies raised against purified human plasminogen were characterized for their effects on the activation of plasminogen and on three enzymic properties of plasmin: (a) thioesterolysis, (b) fibrinolysis, (c) conversion of high-Mr urokinase to its low-Mr form. None of the monoclonal antibodies inhibited plasminogen (plg) activation by urokinase. The monoclonal antibodies characterized in this study fell into three groups. Anti-plg 1 inhibited (a), (b) and (c), while anti-plg 2 inhibited activities (a), (b) and (c) to varying degrees but also formed complexes with plasmin that were stable to sodium dodecyl sulphate. Anti-plg 3 and anti-plg 4 inhibited only activity (c). Selective use of these monoclonal antibodies demonstrated unequivocally that plasmin mediates the activation of the proenzyme form of urokinase-type plasminogen activator. Besides their use in affinity chromatography, therefore, these antibodies are valuable for defining the role of plasmin in the mechanisms of extracellular matrix degradation.
一种针对人纤溶酶原 Kringle 4 区域上的 ε-氨基己酸结合位点的单克隆抗体，可加速尿激酶对 Glu1-纤溶酶原的激活。
影响因子：
3.9
作者：
Cummings,HS;Castellino,FJ
通讯作者：
Castellino,FJ
针对人 Glu1-纤溶酶原离散区域的单克隆抗体。
影响因子：
2.9
作者：
Ploplis,VA;Cummings,HS;Castellino,FJ
通讯作者：
Castellino,FJ