Rapid splicing and stepwise processing of a transcript from the psbB operon in tobacco chloroplasts: Determination of the intron sites in petB and petD
Rapid splicing and stepwise processing of a transcript from the psbB operon in tobacco chloroplasts: Determination of the intron sites in petB and petD
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烟草叶绿体中 psbB 操纵子转录物的快速剪接和逐步处理：petB 和 petD 中内含子位点的测定
DOI：
10.1007/bf00331145
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
M. Sugiura
中科院分区：
文献类型：
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作者：
Minoru Tanaka;J. Obokata;J. Chunwongse;K. Shinozaki;M. Sugiura
研究了psbB基因簇在烟草叶绿体中的表达。该簇依次包含光系统II的51 kDa叶绿素a脱辅基蛋白（psb B）和10 kDa磷蛋白（psbH）以及细胞色素b6（petB）和细胞色素B/f复合物的亚基IV（PetD）的基因。北方印迹杂交和逆转录分析表明，petB和petD含有单个内含子和psbB基因簇转录为一个单一的多顺反子单位。初级转录物似乎非常迅速地剪接，然后加工成几种小RNA。确切的剪接位点已通过cDNA测序定位。psbB操纵子的转录起始位点已被确定通过S1映射与体外加帽叶绿体RNA。对叶绿体RNA前体的分步加工进行了讨论。
SummaryExpression of the psbB gene cluster in tobacco chloroplasts has been studied. This cluster contains the genes for the 51 kDa chlorophyll a apoprotein (psbB) and the 10 kDa phosphoprotein (psbH) of photosystem II, and cytochrome b6 (petB) and subunit IV (PetD) of the cytochrome b/f complex in this order. Northern blot hybridization and reverse transcription analyses have revealed that petB and petD contain single introns and the psbB gene cluster is transcribed as a single polycistronic unit. The primary transcript seems to be spliced very rapidly and then processed into several small RNA species. The exact splice sites have been located by cDNA sequencing. The transcriptional initiation site of the psbB operon has been determined by S1 mapping with in vitro capped chloroplast RNA. The stepwise processing of chloroplast RNA precursors is discussed.
Mol.Gen.Genet.202-2。
DOI：
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发表时间：
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期刊：
影响因子：
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作者：
通讯作者：
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影响因子：
11.4
作者：
SHINOZAKI, K;OHME, M;SUGIURA, M
通讯作者：
SUGIURA, M